Preparation and isolation of single-stranded biotinylated nuclei

Details Preparation and isolation of single-stranded biotinylated nuclei Preparation and isolation of single-stranded biotinylated nuclei

1990-05-04

1995-02-07

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 536 187, 536 231, 536 243, C12D 108, C07H 504, C07H 1700

Patent

active

053875052

ABSTRACT:
A biotinylated target nucleic acid can be isolated from a mixture of nucleic acids using a capture reagent comprising avidin. The targeted nucleic acid is complexed with avidin on a substrate and separated from uncomplexed materials. The complex is heated at above about 65.degree. C. for a suitable time to cleave the avidin-biotin bonds to release the desired targeted nucleic acid. Upon release, the targeted nucleic acid can be collected or detected in a suitable manner, such as with a complementary probe. This method is also useful for preparing single-stranded DNA for a number of uses.

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