Method for determining lipids

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 11, 435 18, 435 19, 435 28, 435803, 436 71, 436907, C12Q 126, C12Q 134, C12Q 128, G01N 3392

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active

047849452

ABSTRACT:
A method for determining lipids using thin layer chromatography and multiple enzymatic staining is described. The method uses a hydrolase enzyme, an oxidase enzyme and a peroxidase enzyme in the presence of a chromogen precursor to form an oxidized chromogen stain. The method is particularly useful in determining phospholipids from animal and human tissue and/or fluids.

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