Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1992-12-11
1995-01-31
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435 691, 4353201, 536 241, C12N 1500, C12N 121, C12N 1511
Patent
active
053858417
ABSTRACT:
There is disclosed a new calcium-regulated promoter to be used for increasing production of extracellular enzymes, or heterologous polypeptides, a recombinant vector that includes the DNA sequence of the promoter operatively linked to a DNA encoding said enzyme or polypeptide and a host organism transformed with the recombinant vector that includes the promoter operatively linked to the DNA encoding said enzyme or polypeptide. The present invention further relates to Streptomyces expression systems and methods for expressing foreign DNA sequences in Streptomyces and for secreting to the surrounding medium polypeptides and proteins coded for by those foreign DNA sequences.
REFERENCES:
patent: 4766066 (1988-08-01), Kuhstoss et al.
Berman et al. 1985 Gene 37:101-110.
J. M. Ward et al., "Construction and charicterisation of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator", Mol. Gen. Genet. (1986) 203:468-478.
M. Geistlich et al., "Localization and functional analysis of the regulated promoter from the Streptomyces glaucescens mel operon", Cell (1989) 3:1061-1069.
E. Katz et al., "Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces limidans", J. Gen. Microbiol. (1983) 129:2703:2714.
W.-M. Leu et al., "Analysis of the promoter region of the melanin locus from Streptomyces antibioticus", Gene (1989) 84:267-277.
Garcia Tomas V.
Gil Jose A.
Martin Juan F.
Ortega Jose D.
Brown Gary L.
Laboratorios Serono S.A.
Schwartz Richard A.
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