Method of isolating lung cell line

Details Method of isolating lung cell line Method of isolating lung cell line

1993-05-11

1994-11-15

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 4, 435 6, 435 29, 435 32, 4351721, 4351722, 4351723, 435240, 435 31, 435 701, C12N 500, C12N 1500, C12P 2102, C12Q 100

Patent

active

053647858

ABSTRACT:
A novel bronchial or bronchiolar epithelial cell from normal neonatal rat lung has been isolated, established and maintained for multiple passages in the absence of serum, without undergoing crisis or senescence. By careful manipulation of the nutritional/hormonal microenvironment we have been able to select, from a heterogeneous population, a single epithelial cell type which can maintain highly differentiated features in vitro. This cell type has characteristics of bronchiolar epithelial cells. A clonal line, RL-65, has been selected and observed for more than 2 years in continuous culture. It has been characterized by ultrastructural, morphological and biochemical criteria.

REFERENCES:
patent: 4885238 (1989-12-01), Reddel et al.
Freshney, "Bronchial and tracheal epithelium", Culture of Animal Cells, A Manual of Basic Technique, Alan R. Liss, Inc., New York, USA (1987).
Celis et al., Electrophoresis 1989, 10:76-100.
Lechner et al., In vitro, 18:633-642 (1982).
Lechner et al., Journal of Tissue Culture Methods, 9:43-48 (1985).

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