Method for simultaneously monitoring turnover rate in multiple p

Details Method for simultaneously monitoring turnover rate in multiple p Method for simultaneously monitoring turnover rate in multiple p

1984-02-27

1985-04-02

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

424 11, 435 35, C12Q 129, C12Q 116, G01T 100

Patent

active

045088209

ABSTRACT:
The invention provides an assay method for cellular and extracellular protein biodynamics comprising the use of radiochemical techniques in combination with high resolution protein separation procedures and recently developed silver stain techniques, to assess turnover of polypeptides over time. Quenching of polypeptide radiolables in silver stained gels is obviated by the use of .sup.14 C-labelled amino acids as precursors.

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Merril et al., Proc. Natl. Acad. Sci., U.S.A., 76, (9), 4335-4339, (1979).
Merril et al., Science, 211, (4489), 1437-8, (1981).
Van Keuren et al., Analyt. Biochem., vol. 116, (1981), 248-255.
Mafart et al., Chem. Abstracts, vol. 94, (1981), #135432d.
Kucukalic et al., Chem. Abstracts, vol. 89, (1978), #176958v.
Milenkovic, Chem. Abstracts, vol. 90, (1979), #85652v.
"Variations in Expression of Mutant .beta. Actin Accompanying Incremental Increases in Human Fibroblast Tumorigenicity", Cell, 28: 259-268, (1982).

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