Detection of endotoxins of gram negative bacteria

Details Detection of endotoxins of gram negative bacteria Detection of endotoxins of gram negative bacteria

1989-12-08

1991-10-22

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 34, C12Q 102

Patent

active

050595270

ABSTRACT:
A method and apparatus for inferring and quantifying the presence and identifying endotoxins of Gram-negative bacteria in a bacteria-containing sample comprising obtaining a sample, extracting any lipids from the sample using supercritical CO.sub.2, hydrolyzing the lipid insoluble residue with a mild acid catalyst, derivatizing the hydroxy fatty acids, and analyzing the sample using a gas chromatograph and mass spectrometer. The results of the gas chromatograph and mass spectrometer analysis allow detection of as few as 10 bacteria or parts of bacteria in a 100 ml sample and specification of the type of Gram-negative bacterial group.

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