Hybrid plasmid vectors, recombinant plasmids containing genes en

Details Hybrid plasmid vectors, recombinant plasmids containing genes en Hybrid plasmid vectors, recombinant plasmids containing genes en

1994-11-17

1997-08-05

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435136, 4352523, 43525233, 4353201, C12P 1302, C12P 740, C12N 120, C12N 1574

Patent

active

056541805

ABSTRACT:
The present invention provides a recombinant plasmid comprising combining a hybrid plasmid vector with the isolated DNA sequences of one or more genes encoding nitrile degrading enzymes which are derived from bacteria belonging to the genus Rhodococcus, said hybrid plasmid vector comprising an isolated DNA sequence which confers on the vector the ability to replicate and amplify in the cells of bacteria belonging to the genus Rhodococcus, and an isolated DNA sequence which confers on the vector the ability to replicate and amplify in the cells of bacteria belonging to Escherichia coli, and an isolated DNA sequence containing a drug resistance gene.

REFERENCES:
patent: 4920054 (1990-04-01), Kozlowski et al.
patent: 5246857 (1993-09-01), Yu et al.
Singer et al. (Feb. 1988), J. Bacteriol. 170(2): 638-645.
Ikehata et al. (1989), Eur. J. Biochem. 181: 563-570.
Hashimoto et al. (1991), Biochim Biophys. Acta 1088 (2): 225-233 [Chem. Abstracts 115, No. 107240y (1991)]Abstract only is provided.

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