Polynucleotides encoding human mitochondrial chaperone proteins

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 6, 4353201, 435325, 4352523, 536 231, 536 2431, 530350, C12P 2106, C12Q 168, C07H 1700, C07K 1400

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060108797

ABSTRACT:
The present invention provides a human mitochondrial chaperone protein (Hmt-GrpE) and polynucleotides which identify and encode Hmt-GrpE. The invention also provides expression vectors and host cells and a method for producing Hmt-GrpE. The invention also provides for antibodies or antagonists specifically binding Hmt-GrpE, and their use in the prevention and treatment of cancer. The invention also provides diagnostic assays. The invention also provides for the use of Hmt-GrpE in identifying antifungal and antiprotozoal therapeutics.

REFERENCES:
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Pfanner, N. et al., "Pulling in the proteins", Curr. Biol., 5: 132-135 (1995).
Laloraya, S. et al., "A role for a eukaryotic GrpE-related protein, Mgelp, in protein translocation", Proc. Natl. Acad. Sci. USA, 91: 6481-6485 (1994) (GI 493576).
Webster, T.J. et al., "cDNA Cloning and Efficient Mitochondrial Import of Pre-mtHSP70 from Rat Liver", DNA and Cell Biol., 13: 1213-1220 (1994).
Naylor, D.J. et al., "Isolation and characterisation of a cDNA encoding rat mitochondrial GrpE, a stress-inducible nucleotide-exchange factor of ubiquitous appearance in mammalian organs", Febs Letts., 396: 181-188 (1996) (GI 1695878).
Lee, J.Y. et al., (Direct Submission), GenBank Sequence Database (Accession 992710), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894 (GI 992710).
Conboy, J.G. et al., "Processing of Pre-Ornithine Transcarbamylase Requires A Zinc-Dependent Protease Localized to the Mitochondrial Matrix", Biochem. Biophys. Res. Comm., 105: 1-7 (1982).
Naylor, D.J. et al., (Direct Submission), GenBank Sequence Database (Accession 1695878), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894 (GI 1695878).
Naylor, D.J. et al., (Direct Submission), GenBank Sequence Database (Accession U62940), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894 (GI 1695877).
(Direct Submission), GenBank Sequence Database (Accession 493576), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894 (GI 493576).
Laloraya, S. et al., (Direct Submission), GenBank Sequence Database (Accession U09565), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894 (GI 493575).
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