Methods of preparing and recovering proteins

Details Methods of preparing and recovering proteins Methods of preparing and recovering proteins

1996-11-20

2000-01-04

Hutzell, Paula K.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 71, 435 771, 435 792, 530350, 530344, 530361, C12Q 100, G01N 3353, A61K 3800, C07K 100

Patent

active

060108444

ABSTRACT:
There is disclosed the preparation and recovery of proteins, in particular of enzymes, by controlled proteolytic cleavage of pro-proteins, in particular pro-enzymes, in a single method step, wherein a pro-protein-containing solution is contacted with a protease and with a solid carrier which has a higher affinity to the protein than to the pro-protein or to the functionally inactive degradation products thereof, the pro-protein being proteolytically cleaved to said protein, and the protein being selectively separated by absorption on the solid carrier.

REFERENCES:
patent: 5053453 (1991-10-01), Ku
patent: 5296352 (1994-03-01), Schlaeppi
Burgess et al. (J. Cell Bio, 111:2129-2138), 1990.
Lazar et al. (Mol & Cell Bio, 8:1247-1252), 1998.
Tero et al (J. Immunol,143:2595-2601), 1989.

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