Process for producing an insulin

Details Process for producing an insulin Process for producing an insulin

Filed on

1981-05-11

Date issued

1983-12-20

Examiner

Waddell, Frederick E.

Patent Class

Chemistry of carbon compounds

SubClass

Miscellaneous organic carbon compounds

SubSubClass

C-metal

Other Related Categories

C07C10352, C07G 700

Type

Patent

Status

active

Patent number

044216858

Description

ABSTRACT:
Production of insulin or an insulin analog is provided by combination of an A-chain and a B-chain, which comprises bringing the S-sulfonated form of the A-chain, the S-sulfonated form of the B-chain, and a thiol reducing agent together in an aqueous medium under conditions which produce a mixture having (1) a pH of from about 8 to about 12, (2) a total protein concentration of from about 0.1 to about 50 milligrams per milliliter, and (3) an amount of thiol reducing agent which affords a total of from about 0.4 to about 2.5 --SH groups per each --SSO.sub.3.sup.- group present in the total amount of A- and B-chain S-sulfonates, and allowing formation of insulin or an insulin analog to occur by maintaining the mixture at a temperature of from about 0.degree. C. to about 25.degree. C. and in an environment which provides a source of oxygen.

REFERENCES:
patent: 3420810 (1969-01-01), Katsoyannis et al.
Katsoyannis, Diabetes 13 Jul.-Aug. 1961, 339-348.
Scientia Sinica, 10, 84-104 (1961); 14, 229-236 (1965); 15 544-561 (1966).
Dixon et al., Nature Nov. 26, 1960 721-724.
Kexue Tongbao, (Republic of China) 17 241-277, (1966).
Science, 154, 1509-1514, (1966), Katsoyannis.
Dixon-Symposium 58, pp. 1207-1215 "Recombination of Insulin A and B Chains, Hybrid Insulins and Synthetic Insulin".
Dixon-Biochimica et Biophysica Acta (1962) 483-489.

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