Process for the release of poly(3-hydroxy carboxylic acids)

Details Process for the release of poly(3-hydroxy carboxylic acids) Process for the release of poly(3-hydroxy carboxylic acids)

1990-12-26

1992-09-22

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C12P 742

Patent

active

051496445

ABSTRACT:
Process for releasing poly(3-hydroxy carboxylic acid) granules from cells of Gram-negative bacteria containing the latter, by expression of a cloned natural or chimeric lysis gene, in which, before induction of lysis gene expression, the ionic strength of doubly charged cations is increased to between 0.05 and 0.5 mol/1, the expression of the lysis gene is induced by raising the temperature, the cells are harvested, and the still wet cells are resuspended in water or buffer solutions, which results in the release of the polymer granules by spontaneous lysis.

REFERENCES:
patent: 4477655 (1984-10-01), Holmes
patent: 4520016 (1985-05-01), Hultmark et al.
Slater et al., "J. Bacteriol", 170 (10) 4431-4436 (1988).
Witte et al., "Eur. J. Biochem.", 180 393-398 (1989).
Blasi et al., "J. Gen. Microbiol.", 131 1107-1114 (1985).
Chang et al., "J. Bacteriol.", 1141-1156 (Jun. 1978).

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