Method for introducing cloned, amplifiable genes into eucaryotic

Details Method for introducing cloned, amplifiable genes into eucaryotic Method for introducing cloned, amplifiable genes into eucaryotic

1988-09-26

1992-09-22

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 692, 435 6, 4351723, 4352402, 4352404, 4353201, 435811, 435948, 435 693, 435 694, 435 6951, 435 696, 435 91, 435191, 435193, 435194, 435255, 514 2, 514 3, 514 4, 514 23, 935 4, 935 27, 935 31, 935 42, 935 56, 935 58, 935 66, C12P 2102, C12Q 168, C12N 1510

Patent

active

051496364

ABSTRACT:
Multiple copies of a foreign DNA I coding for the production of a protein may be introduced into eucaryotic cells by cotransforming suitable cells with foreign DNA I and with foreign DNA II which includes a functionally deficient, amplifiable gene coding for a selectable or identifiable trait, preferably carried on the same DNA molecule as a foreign DNA III, which includes a functional, amplifiable gene coding for another selectable or identifiable trait. The cotransformation is carried out under suitable conditions permitting selection or identification of cells expressing the gene on DNA I or that on DNA III, but not that on DNA II. The cotransformed cells so identified or selected are recovered and cloned under conditions where the functionally deficient gene on DNA II is expressed. Cells expressing the gene on DNA II are recovered. They contain multiple copies of DNA I. This method can be used to produce mRNA transcripts or protein products such as human and animal growth hormone, insulin and the like.

REFERENCES:
patent: 4399216 (1983-08-01), Axel
patent: 4634665 (1987-01-01), Axel

Affiliated with

Also associated with

Rating

Method for introducing cloned, amplifiable genes into eucaryotic does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for introducing cloned, amplifiable genes into eucaryotic, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for introducing cloned, amplifiable genes into eucaryotic will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1067900