Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1990-05-16
1992-09-22
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 4351723, 4352402, 935 34, 935 60, 935 70, C12P 2102, C12N 1585, C12N 510, C12N 1500
Patent
active
051496356
ABSTRACT:
Disclosed is a method of increasing production of proteins, e.g., human tPA, in mammalian cells which normally secrete immunoglobulins. Degradation of mRNA transcribed from recombinant DNA is decreased by decreasing the length of the untranslated region of the mRNA. The untranslated region of DNA encoding a protein of interest is altered to produce a shorter recombinant DNA having an untranslated region comprising a poly A addition signal (AATAAA) and less than about 300 nucleotide bases interposed between said poly A signal and the stop codon 3' of the coding region of the gene of interest. The mammalian cell line is transfected and cultured to produce greater amounts of the protein of interest than the same cell line transfected with the unaltered DNA.
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Abbott Biotech, Inc.
Nolan S. L.
Schwartz Richard A.
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