Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1979-05-03
1981-11-03
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435188, 435810, 23230B, 424 12, C12N 996, G01N 3354
Patent
active
042986850
ABSTRACT:
A sample, e.g. serum, containing an antigen, hapten or other biological substance is mixed with antibodies raised against that substance which have been tagged with biotin, and with a known amount of that substance labelled with an enzyme. After the competitive complexation of the antibody with the labelled substance and the substance to be detected, avidin, immobilised on an inert support is added. The avidin binds to the biotin and causes the complex to be precipitated. The solid and liquid phases are separated by centrifugation and the amount of biological substance in the original sample is determined by measuring the activity of the enzyme in either phase.
REFERENCES:
patent: 3839153 (1974-10-01), Schuurs et al.
patent: 3852157 (1974-12-01), Rubenstein et al.
patent: 4017597 (1977-04-01), Reynolds
patent: 4134792 (1979-01-01), Boguslaski et al.
May et al. "NEO29-(t)-Biotinyl insulin and its Complexes with Avidin", J. Biol. Chem., vol. 253, No. 3 (1978) pp. 686-690.
Manning et al. "A Method for Gene Enrichment Based on the Avidin-Biohn Interaction Application to the Drosophilia Ribosoma RNA Genes", Biochem., vol. 16 No. 7 (1977) pp. 1364-1370.
Heggeness et al. "Use of the Avidin-Biotin Complex for the Localization of Actin and Myosin with Fluorescence Microscopy", J. Cell Biol., vol. 73 (1977) pp. 783-788.
Hofmann et al. "Avidin-Biotin Affinity Columns, General Methods for Attaching Biotin to Peptides and Proteins", J. Am. Chem. Soc., vol. 100, No. 11 (1978) pp. 3585-3590.
Bayer, et al. "Preparation of Ferriton-Avidin Conjugates by Reductive Alkylation for Use in Electron Microscopic Cytochemistry", J. Hist. Cytochem., vol. 24, No. 8 (1976) pp. 933-939.
Cuatrecasas Pedro
Parikh Indu
Brown Donald
Burroughs Wellcome Co.
Wiseman Thomas G.
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