Double antibody separation method

Details Double antibody separation method Double antibody separation method

1979-04-05

1981-11-03

Hunt, Brooks H.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 422 61, 424 12, 435 7, 435 17, 435 21, G01N 3348, G01T 100, A61K 4300, B65D 7100

Patent

active

042985927

ABSTRACT:
A double antibody radioimmunoassay method for determining antigens in a sample wherein polyethylene glycol is provided in a reaction medium to accelerate an immunoprecipitation reaction between water-soluble first antibodies bound to antigen and water-soluble second antibodies which are not specific to said antigens but which are specific to said first antibodies. A double antibody, multi-component radioimmunoassay test kit is also provided. The kit contains radiolabeled antigen reagent, first water-soluble antibody reagent and second water-soluble antibody reagent, as well as a polyethylene glycol reagent, which may optionally be combined with the second antibody reagent, in an amount sufficient to accelerate an immunoprecipitation reaction, between said first and second reagents.

REFERENCES:
patent: 3995019 (1976-11-01), Jerome
patent: 4018883 (1977-04-01), Parslow
patent: 4139604 (1979-02-01), Gutcho et al.
Chard, An Introduction to Radioimmuno Assay and Related Techniques, North Holland Pub. Co., Amsterdam, 1978, pp. 410-417.
Remes et al., Radio Chem. Radioanal Letters, 34(2-4),pp. 253-260 (1978).
Blank-uss et al, Clinica Chimica Acta, 86, pp. 67-72 (1978).

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