Production of vitamin B.sub.6 with an enzyme-containing cell ext

Details Production of vitamin B.sub.6 with an enzyme-containing cell ext Production of vitamin B.sub.6 with an enzyme-containing cell ext

1999-04-14

2000-05-09

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

426311, 435170, 435183, 435822, A23L 130, A61K 5100, C12N 100, C12P 100, C12P 104

Patent

active

060602678

ABSTRACT:
A process for the enzymatic production of vitamin B.sub.6 which includes incubating 1-deoxy-D-threo-pentulose and 4-hydroxy-L-threonine with an enzyme system that is cell free or essentially cell free and prepared from the cells of a microorganism belonging to the genus Rhizobium, Sinorhizobium, Flavobacterium, Chryseobacterium, Lactobacillus, Arthrobacter, Bacillus, Klebsiella, Escherichia, Pseudomonas, Stenotrophomonas, Enterobacter, Serratia, Corynebacterium, Brevibacterium, Exiguobacterium, Saccharomyces, Yamadazyma, Pichia or Candida, in the presence of NADP.sup.+, NAD.sup.+, ATP. Manganese and magnesium ions stimulate the above reaction. This process affords high yields of vitamin B.sub.6, a vitamin essential for the nutrition of animals, plants and microorganisms, and which is also useful as a medicine or food additive. In addition, an enzyme reaction system for producing vitamin B.sub.6 and a process for making the enzyme reaction system are also provided.

REFERENCES:
patent: 5766894 (1998-06-01), Hoshino et al.
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Nishio, N., et al., Utilization of n-Paraffins and Vitamin B.sub.6 Production by Pichia guilliermondii Wickerham, Agr. Biol. Chem., 37(3), pp. 553-559, (1973).
Suzue, R., et al., "Biosynthesis of Vitamin B.sub.6 " The Journal Of Vitaminology 16, pp. 154-159, (1970).
Ishida, M., et al., "Studies on the Biosynthesis of Vitamin B.sub.6 " Agr. Biol. Chem., vol. 34 No. 4, pp. 327-334 (1970).
Pflug, W., et al., "Vitamin B.sub.6 Biosynthesis in Bacillus Subtilis" Hoppe Seyler's Z. Physiol. Chem. Bd. 359, 559-570 (1978).
Hill, R.E., et al., "The Biogenetic Anatomy of Vitamin B.sub.6 " The Journal of Biological Chemistry vol. 271, No. 48, pp. 30426-30435 (1996).
Hill, R.E., et al., "Biosynthesis of Vitamin B.sub.6 " in Escherichia coli and Salmonella 2nd ed., F.C. Neidhardt, Ed., pp. 695-703 (1996).

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