Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1994-11-10
1996-08-27
Wax, Robert A.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
43525411, 4352523, 4353201, 536 235, C12N 121, C12N 115, C12N 510, C12N 1512, C12N 1563
Patent
active
055500498
ABSTRACT:
DNAs encoding voltage-activated cation channels have been cloned and characterized. The cDNA's have been expressed in recombinant host cells which produce active recombinant protein. The recombinant protein is also purified from the recombinant host cells. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the channel activity, and channel modulators are identified. Channel modulators are useful as insecticides and arachnicidic agents.
REFERENCES:
Hall and Kasbekar, Drosophila Sodium Channel Mutations Affect Pyrethroid Sensitivity, in Insectide Action, (eds. Narahashi et al.) New York: Plenum Press pp. 99-114. 1981.
Noda, Masaharu et al., Existence of distinct sodium channel messenger RNAs in rat brain, Nature, vol. 320, pp. 188-192 (1986).
Liman, E., et al., Subunit Stoichiometry of a Mammalian K+ Channel Determined by Construction of Multimeric cDNAs, Neuron, vol. 9, pp. 861-871 (1992).
Jackson, F., et al., The tip-E Mutation of Drosophila Decreases Saxitoxin Binding and Interacts with Other Mutations Affecting Nerve Membrane Excitability, Journ. of Neurogenetics, 3, pp. 1-17 (1986).
Taylor, Martin F. J. et al., Linkage of Pyrethroid Insecticide Resistance to a Sodium Channel Locus in the Tobacco Budworm, Insect Biochem. Molec. Biol., vol. 23, No. 7, pp.763-775, (1993).
Knipple, D. C., et al., Tight genetic linkage between the kdr insecticide resistance trait and a voltage-sensitive sodium channel gene in the house fly, Proc. Natl. Acad. Sci., vol. 91, pp.2483-2487 (1994).
Williamson, M., et al., Knockdown resistance (kdr) to DDT and pyrethroid insecticides maps to a sodium channel gene locus in the housefly (Musca domestica), Mol. Gen. Genet. 240: pp. 17-22 (1993).
Hall, L. M. et al., Molecular and genetic analysis of tip-E: a mutation affecting sodium channels in Drosophila, 35th Annual Drosophila Res. Conf., Program & Absts. p. 77. (1994).
Hall & Feng, Abstracts of papers presented at the 48th Annual mtg of the society of general physiologists, Marine Biological Lab, The tip-E Locus Defines a Novel Membrane Protein Required During Development to Rescue Adult Paralysis, p. 11a, (1994).
O'Dowd and Aldrich, Voltage-Clamp Analysis of Sodium Channels in Wild-type and Mutant Drosophila Neurons, The Journal of Neuroscience, 8 (10), p. 3633-3643 (1988).
Barry Ganetyzky, Neurogenetic Analysis of Drosophila Mutations Affecting Sodium Channels: Synergistic Effects on Viability and Nerve Conduction in Double Mutants Involving tip-E, Journal of Neurogenetics, 3, pp. 19-31 (1986).
Thackeray and Ganetzky, Developmentally Regulated Alternative Splicing Generates a Complex Array of Drosophila para Sodium Channel Isoforms, The Journal of Neuroscience, 14 (5), pp. 2569-2578 (1994).
Loughney, K., et al., Molecular Analysis of the para Locus, a Sodium Channel Gene in Drosophila, Cell, vol. 58, pp. 1143-1154 (1989).
Ramaswami and Tanouye, Two sodium-channel genes in Drosophila: Implications for channel diversity, Proc. Natl. Acad. Sci., vol. 86, pp. 2079-2082 (1989).
Salkoff, L., et al., Genomic Organization and Deduced Amino Acid Sequence of a Putative Sodium Channel Gene in Drosophila, Science, vol. 237 pp. 744-749 (1987).
Gordon, D., et al., Biochemical Characterization of Insect Neuronal Sodium Channels, Archives of Insect Biochemistry and Physiology 22: pp. 41-53 (1993).
William A. Catterall, Cellular and Molecular Biology of Voltage-Gated Sodium Channels, Physiological Reviews, vol. 72, No. 4 (Suppl.) pp. S15-S48 (1992).
Thummel, C. et al., Vectors for Drosophila P-element-mediated transformation and tissue culture transfection, Gene, 74, pp. 445-456 (1988).
Bunch, T., et al., Characterization and use of the Drosophila metallothionein promoter in cultured Drosophila melanogaster cells, Nucleic Acids Research, vol. 16, No. 3, pp. 1043-1059 (1988).
Noda, M., et al., Primary Structure of Electrophorus Electricus Sodium Channel Deduced from cDNA Sequence, Nature, vol., 312, 8, pp. 121-127 (1984).
Stevens, Charles, And now the sodium channel, Nature, vol. 312, pp. 98-99 (1984).
Van Der Ploeg Leonardus H. T.
Warmke Jeffrey W.
Bugalsky Gabriele E.
Merck & Co. Inc
Tribble Jack L.
Wax Robert A.
LandOfFree
Process for identifying para cation channel modulators does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for identifying para cation channel modulators, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for identifying para cation channel modulators will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1055859