Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-01-15
1994-07-26
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
436164, 436172, 935 77, 935 78, C12Q 168, G01N 2164
Patent
active
053326598
ABSTRACT:
A target polynucleic acid strand may be detected within a sample. A sensing strand of complementary polynucleic acid is labelled, at at least two internucleotide phosphate groups, with labels which vary their electronically interaction with each other, and thus their emission or absorption spectra, depending upon whether the sensing strand is bound to target polynucleic acid in the sample.
REFERENCES:
Sambrook et al. Molecular Cloning: A Laboratory Manual, 1989, Cold Springs arbor Laboratory Press.
Forster et al. Angew Chem. Intern Edit (1969) 8:333-343.
Morrison, "Time-Resolved Detection Of Energy Transfer: Theory And Application To Immunoaasays," Analytical Biochemistry, 1988 pp. 101-120.
Chandross et al., "Intramolecular Excimer Formation And Fluorescence Quenching In Dinaphthylalkanes," Journal of the American Chemistry Society, 1970, pp. 3586-3593 .
Cuniberti et al., "Intramolecular Excimer Formation In Polymers," European Polymer Journal vol. 16, 1980, pp. 887-893.
Patel et al., "Homogeneous Immunoassay Based on Chemiluminescence Energy Transfer," Clin. Chem. 29/9, 1604-1608, 1983.
Pohl et al., "Chromophoric And Fluorophoric Peptide Substrates Cleaved Through The Dipeptidyl Carboxypeptidase Activity Of Cathepsin B," Analytical Biochemistry 165, 96-101, 1987.
Carmel et al., "Use Of Substrates With Fluorescent Donor And Acceptor Chromophores For The Kinetic Assay Of Hydrolases," North-Holland Publishing Company-Amsterdam, vol. 30 No. 1, Febs Ltrs., 1973, pp. 11-14.
Telser et al., "DNA Duplexes Covalently Labeled At Two Sites: Synthesis And Characterization By Steady-State And Time Resolved Optical Spectroscopies," J. Am. Chem. Soc., 1989, pp. 7226-7232.
Edelberg Barry A.
McDonnell Thomas E.
Myers Carla
Parr Margaret
The United States of America as represented by the Secretary of
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