Hybrid viral plasmid and microorganisms containing same

Details Hybrid viral plasmid and microorganisms containing same Hybrid viral plasmid and microorganisms containing same

1983-05-19

1985-12-31

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435240, 435253, 435317, 935 24, 935 29, 935 32, C12N 1500, C12N 500, C12N 120, C12N 100

Patent

active

045621551

ABSTRACT:
pML BK is a hybrid plasmid which is constructed of :pML which contains the entire nucleotide sequence of the plasmid pBR322 except for deletion of nucleotide sequences 1120-2490 bp and 0 to 375 bp, and BK which contains the entire nucleotide sequence of the Gardner strain of BK virus except for deletion of nucleotide sequences between bp 5089-5196. Into the pML BK plasmid may be inserted a viral or human gene X to be expressed, an example of which is TK which represents the Bam HI generated 3,600 bp DNA fragment of herpes simplex virus type 1 which contains the herpes simplex virus type 1 TK DNA. The majority of the pML BK X DNA molecules remain in the episomal or free state in the human cells 143B and express the gene X.

REFERENCES:
Binetruy et al., Eukaryotic Viral Vectors, Cold Spring Harbor Laboratory, pp. 87-92, (1982).
Kareh et al., Somstic Cell Genetics, Plenum Press, pp. 111-125, (1981).
De Pamphilis et al., Organization and Replication of Viral DNA, CRC Press Inc., pp. 37-114, (1982).
DiMaio et al., PNAS, vol. 79, pp. 4030-4034, Jul. 1982.
Gething et al., Nature, vol. 293, pp. 620-625, Oct. 1981.
Gruss et al., PNAS, vol. 78, pp. 133-137, Jan. 1981.
Lusky et al., Nature, vol. 293, pp. 79-81, (1981).
Bolivar et al., Gene 2, pp. 95-113, (1977).
Gardner et al., vol. 1, pp. 1253-1257, (1971).
McKnight et al., Carnegie Institute Year Book, vol. 78, pp. 56-61, (1978).

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