Method of measuring biological ligand

Details Method of measuring biological ligand Method of measuring biological ligand

1984-04-24

1987-03-10

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 7, 435 15, 435188, 436518, 436531, 436813, 436817, G01N 33543, G01N 33545, G01N 33569, G01N 33574

Patent

active

046491058

ABSTRACT:
A method of measuring a biological ligand comprises allowing to coexist a biologically active composition comprising an immobilization phase of a particular antibody or a particular ligand and an immobilization phase of a biotinyl enzyme or a biotinyl enzyme inhibitor, a water-soluble conjugate of the ligand or the antibody and the biotinyl enzyme inhibitor or the biotinyl enzyme, and the ligand to be measured in an aqueous solution, and measuring the remaining biotinyl enzyme activity or the biotinyl enzyme inhibitory activity. This method is highly sensitive and specific, and it is suitable as a clinical test for the determination of physiological substances and trace components in humoral fluid.

REFERENCES:
patent: 4208479 (1980-06-01), Zuk et al.

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