Apparatus for the pure preparation of particles, biological cell

Details Apparatus for the pure preparation of particles, biological cell Apparatus for the pure preparation of particles, biological cell

1985-02-06

1987-03-10

Jenkins, Robert W.

Imperforate bowl: centrifugal separators

Including plurality of miniature bowls distrubuted about...

And means for introducing either material or auxiliary fluid...

422 72, 436 45, 494 13, B04B 502

Patent

active

046488634

ABSTRACT:
A process for the pure preparation of particles, biological cell systems, colloids and the like, in which liquid density gradients are produced in a rotating centrifuge container (1), and in which the particles and the like are introduced into the centrifuge container (1), individual bands being produced in such a manner that the bands containing the heavier particles are more remote from the axis of rotation than are the bands containing the lighter particles. The density gradients are constructed by the introduction of fractions of different density (a-f) via a cannula (18) which extends into the centrifuge container (1) to just before a tip (19) of the latter, the lightest fraction (a) being introduced first and the heaviest fraction (f) being introduced last. After the particles and the like have been introduced and the individual bands (a' to f') have been produced, the individual bands (a' to f') are removed via the cannula (18) by pumping out from the centrifuge container (1) which has been rotated at a preset separating speed of rotation.

REFERENCES:
patent: 2616619 (1952-11-01), Leod
patent: 3452924 (1969-07-01), Schlutz
patent: 3679367 (1972-07-01), Negersmith
patent: 4456581 (1984-06-01), Edelmann

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