Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-01
1997-10-21
Ketter, James
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 691, 4351723, 435189, 536 232, 536 235, C12Q 168, C12P 2102, C12N 1500, C12N 902
Patent
active
056795211
ABSTRACT:
Disclosed are methods and compositions for the preparation of steroid 5.alpha.-reductases by recombinant means, as well as for the use of these enzymes in screening assays for the identification of compounds which have the ability to inhibit or otherwise alter the enzymatic function of these enzymes. Biochemical and pharmacological evidence is presented to demonstrate the existence of more than one human steroid 5.alpha.-reductase. The DNA sequence encoding steroid 5.alpha.-reductase 2, the major active isozyme of human genital tissue, is disclosed herein, in addition to methods and compositions for its preparation and pharmacological analysis. The sequences disclosed herein may be used directly in the preparation of genetic constructs, or may be employed in the preparation of hybridization probes for the selection of enzyme-encoding sequences from other sources. These sequences may prove useful in an analysis of normal and abnormal sexual differentiation, benign prostatic hyperplasia, male pattern baldness, acne, hirsutism, endometriosis, and cancer of the prostate.
REFERENCES:
patent: 5422262 (1995-06-01), Andersson et al.
Montell and Goodman, "Drosophila Substrate Adhesion Molecule: Sequence of Laminin B1 Chain Reveals Domains of Homology with Mouse", Cell, 53:463-473, 1988.
Thigpen, et al., "Characterization of Chinese Hamster Ovary Cell Lines Expressing Human Steroid 5.alpha.-Reductase Isozymes", Journal of Biological Chemistry, 268:17404-17412, 1993.
Andersson Stefan
Russell David W.
Board of Regents , The University of Texas System
Brusca John S.
Ketter James
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