Lipase activity determining method and reagent

Textiles: fiber preparation

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195 99, C12K 104, C12K 110

Patent

active

039869300

ABSTRACT:
A reagent for determining lipase activity, which comprises as the active ingredient one of the S-acyl compounds of the formula: ##EQU1## WHEREIN N IS AN INTEGER OF 2 TO 4, INCLUSIVE; N' IS AN INTEGER OF 2 TO 4, INCLUSIVE OR OF 9 TO 13, INCLUSIVE; M IS AN INTEGER OF 3, 7 OR 11; M' IS AN INTEGER OF 4 OR 12; Y IS AN INTEGER OF 1 OR 2; X is sulfur atom or oxygen atom; Y is sulfur atom, oxygen atom or ethylene group; provided that when y is 1, m' is 4 and when y is 2, m' is 12, and a method for determining lipase activity by reacting the S-acyl compound with lipase in a buffer solution containing a chromogenic sulfhydryl reagent and then subjecting the resultant to a colorimetry.

REFERENCES:
J. F. Whitaker "A Rapid and Specific Method for the Determination of Pancreatic Lipase in Serum and Urine" Clinica Chimica Acta, 44 (1973) 133-138.
F. P. Doyle et al., "Antituberculous Sulphur Compounds, Part II" J. Chem. Soc. 2660-2665 (1960).

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