Method for the direct measurement of at least one chemical param

Details Method for the direct measurement of at least one chemical param Method for the direct measurement of at least one chemical param

1991-08-13

1993-10-05

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 4, 435291, 435288, 435817, 20415312, 204403, 128635, C12Q 100, A61B 500, C12M 140, C12M 134

Patent

active

052504190

ABSTRACT:
This biosensor comprises a membrane (8), in which an enzyme catalyzing the transformation of a substrate used in the cutaneous metabolism is immobilized, means for detecting and measuring a phenomenon caused by said transformation, which is representative of the concentration of the substrate to be measured, with said substrate being brought into contact with the enzymatic membrane (8), and a measuring cell (13), a wall area of which is formed by the membrane (8) and which is capable of being closed, during the measurement, by a cutaneous covering area (15), with the cell (13) being combined with means for making circulate therein a liquid for placing in solution the substrate to be measured. For the measurement, the biosensor is applied to the skin, the buffer is injected into the cell and, once said cell is filled, the detection and the measurement are carried out, then the buffer is again made to circulate in order to remove the solubilized substrate. This measuring cycle can be repeated without removing the electrode (1). The biosensor is used for measurement of cutaneous L-lactate with a Clark electrode combined with a membrane using L-lactate oxidase.

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