Liquid purification or separation – Processes – Chromatography
Patent
1992-10-09
1993-10-05
Therkorn, Ernest G.
Liquid purification or separation
Processes
Chromatography
210 85, 2101982, 2041801, 204299R, 356366, 356368, 356369, 422 69, 422 70, 436161, 436171, 436177, B01D 1508
Patent
active
052501868
ABSTRACT:
A high angle light scattering detector using classical Rayleigh scattering. A high intensity arc light source, filtered to leave only one wavelength illuminates a flow cell. Through the flow cell, very small particles such as biological proteins flow in solution after separation by HPLC or some other means. A UV detector generates data regarding the weight concentration of the eluting particles and a scattered light detector collecting scattered light at angles of approximately 90.degree. generates a scattered light signal. The incident light intensity is also measured. The average molecular weight is then computed using the scattered and incident light data, the weight concentration data and a simplified mathematical relationship from which the size factor P and the viral coefficients have been eliminated.
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Cunico Robert L.
Dollinger Gavin D.
Kunitani Michael G.
Cetus Corporation
Therkorn Ernest G.
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