Discovery of and method for cloning and producing the PspGI rest

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase


Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0


43525233, 4353201, 536 232, C12N 1555, C12N 922




The present invention relates to the type II restriction endonuclease PspGI, obtainable from Pyrococcus species G-I-H (NEB #906), an enzyme which recognizes the DNA sequence 5' CC(A/T)GG 3' and cleaves before the first C of the recognition sequence to produce a 5 base 5' extension:

patent: 5200333 (1993-04-01), Wilson
Kosykh et al., Molec. Gen. Genet., 178:717-718 (1980).
Mann et al., Gene, 3:97-112 (1978).
Walder et al., Proc. Nat. Acad. Sci., 78:1503-1507 (1981).
Bougueleret et al., Nucl. Acid Res., 12:3659-3676 (1984).
Gingeras and Brooks, Proc. Natl. Acad. Sci. USA 80:402-406 (1983).
Theriault and Roy, Gene, 19:355-359 (1982).
Blumenthal et al., J. Bacteriol., 164:501-509 (1985).
Kiss et al., Nucl. Acid Res., 13:6403-6421 (1985).
Szomolanyi et al, Gene, 10:219-225 (1980).
Janulaitis et al., Gene, 20:197-204 (1982).
Kiss and Baldauf, Gene, 21:111-119 (1983).
Walder et al., (J. Biol. Chem., 258:1235-1241 (1983).
Piekarowicz et al., Nucleic Acid Res. 19:1831-1835 (1991).
Piekarowicz et al., J. Bacteriology, 173:150-155 (1991).
Fomenkov et al., Nucleic Acids Res. 22:2399-2403 (1994).
Lunnen et al., Gene, 74:25-32 (1988).
Raleigh and Wilson, Proc. Natl. Acad. Sci, USA, 83:9070-9074 (1986).
Heitman and Model, J. Bact., 196:3243-3250 (1987).
Raleigh et al., Genetics, 122:279-296 (1989).
Waite-Rees et al., J. Bacteriology, 173:5207-5219 (1991).
Sanger et al. Proc. Natl. Acad. Sci., 74:5463-5467 (1977).
Brown et al., J. Mol. Biol. 140:143-148 (1980).
Fuller, Gene 19:43-54 (1982).
Shimatake and Rosenberg, Nature, 292:128 (1981).
Shine & Dalgarno, Proc. Natl. Acad. Sci. USA, 71:1342-1346 (1974).
Ikemura, J. Mol. Biol., 151:389-409 (1981).
Matsudaira, J. Biol. Chem. 262:10035-10038 (1987).
Looney, et al., Gene 80:193-208 (1989).
Birnboin and Doly, Nucl. Acids Res. 7:1513 (1973).


Say what you really think

Search for the USA inventors and patents. Rate them and share your experience with other people.


Discovery of and method for cloning and producing the PspGI rest does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Discovery of and method for cloning and producing the PspGI rest, we encourage you to share that experience with our community. Your opinion is very important and Discovery of and method for cloning and producing the PspGI rest will most certainly appreciate the feedback.

Rate now


Profile ID: LFUS-PAI-O-1457013

All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.