Discovery of and method for cloning and producing the PspGI rest

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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43525233, 4353201, 536 232, C12N 1555, C12N 922

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active

058495584

ABSTRACT:
The present invention relates to the type II restriction endonuclease PspGI, obtainable from Pyrococcus species G-I-H (NEB #906), an enzyme which recognizes the DNA sequence 5' CC(A/T)GG 3' and cleaves before the first C of the recognition sequence to produce a 5 base 5' extension:

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