Diagnostic test kit and method for determination of chlamydial a

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 72, G01N 33571

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active

050859862

ABSTRACT:
Antigens from chlamydial organisms in specimens can be rapidly and sensitively determined using a polyamide microporous membrane which has surface hydroxy groups. This determination is accomplished by contacting extracted antigen with the polyamide microporous membrane for a sufficient time for the antigen to bind to the membrane. Antigen bound to the membrane is contacted with chlamydial antibody so as to form an immunological complex on the membrane. The presence of the complex on the membrane in then determined as a measure of the amount of chlamydial antigen present in the specimen. The use of this particular membrane improves reagent keeping and reduces background in the assay.

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