Analytical element and method for the determination of creatinin

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435 25, 435 28, 435810, C12Q 134

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048123994

ABSTRACT:
An analytical element can be used for the determination of either creatinine or creatine or both. The element contains creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase, and a leuco dye which is capable of providing a detectable dye in the presence of hydrogen peroxide and a peroxidative substance. The creatine amidinohydrolase is present in a manner such that it is substantially inert to the leuco dye. The creatinine amidohydrolase is present in a rate limiting amount. By measuring the amount of dye formation at particular times during the assay, either or both of the analytes can be determined with the same element.

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