Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
435 911, 435 915, 435 9151, 435 6, 436501, 536 231, 536 253, 935 7, 935 16, 935 17, 935 78, C12P 1934
A method of amplifying a mixture of different-sequence DNA fragments which may be formed from RNA transcription, or derived from genomic single- or double-stranded DNA fragments. The fragments are treated with terminal deoxynucleotide transferase and a selected deoxynucleotide, to form a homopolymer tail at the 3' end of the anti-sense strands, and the sense strands are provided with a common 3'-end sequence. The fragments are mixed with a homopolymer primer which is homologous to the homopolymer tail of the anti-sense strands, and a defined-sequence primer which is homologous to the sense-strand common 3'-end sequence, with repeated cycles of fragment denaturation, annealing, and polymerization, to amplify the fragments. In one embodiment, the defined-sequence and homopolymer primers are the same, i.e., only one primer is used. The primers may contain selected restriction-site sequences, to provide directional restriction sites at the ends of the amplified fragments.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4734363 (1988-03-01), Dattagupta et al.
Byrne, B.C., et al., Nucleic Acids Res. 16(9):4165 (1988).
Engelke, D.R., et al., Proc. Natl. Acad. Sci. USA 85:544-548 (1988).
Erlich, H.A., et al., Nature 331:461-462 (1988).
Kwon-Shi, O., et al., J. Biol. Chem. 282(5):2121-2130 (1987).
Liang, W., and Johnson, J.P., Nucleic Acids Res. 16:3579 (1988).
Rougeon, F., and Mach, B., Proc. Natl. Acad. Sci. USA 73(10):3418-3422 (1976).
Verma, I.M., J. Virol. 26(3):615-629 (1978).
Kalendin et al, Biokhimiia, 47(11):1785-91 (1982).
Kalendin et al, Biokhimiia, 46(9):1576-84 (1981).
Stenesh, J., Biochem Biophys Acta, 475(1):32-41 (1977).
Ruttimann et al, Eur J Biochem, 149(1):41-46 (1985).
Scharf et al. (1986) Science, vol. 233, pp. 1076-1078.
Gaubatz et al. (1985) Biochim. and Biophys. Acta, vol. 825, pp. 175-187.
Gaubatz et al. (1982) J. Theor. Biol, vol. 95, pp. 679-696.
Benson Robert H.
Dehlinger Peter J.
Genelabs Technologies, Inc.
Marschel Ardin H.
Amplification of mixed sequence nucleic acid fragments does not yet have a rating. At this time, there are no reviews or comments for this patent.If you have personal experience with Amplification of mixed sequence nucleic acid fragments, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Amplification of mixed sequence nucleic acid fragments will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2211088