Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1998-02-24
2000-06-06
Smith, Lynette R. F.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 236, 536 242, 800278, 800295, 800298, 435419, 435468, 4351723, A01H 500, A01H 400, C12N 1500, C12N 1529, C12N 1582
Patent
active
060720506
ABSTRACT:
Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a "TATA to start" sequence containing 64% or greater GC content and an synthetic upstream element incorporating several OCS binding motifs and novel flanking sequences. Upstream activating regions (UARs) are also disclosed that can further increase the constitutive transcriptional activity when they are operably linked to said promoter and/or the synthetic upstream element. In particular, the nucleotide sequence of the UAR of the maize Ubi-1 gene is provided and its use in expression cassettes and vectors containing these promoter elements. Cells and plants transformed with these vectors are further provided. These include a transgenic sunflower expressing an exogenous oxalate oxidase gene at a high level under the transcriptional control of a recombinant promoter having at least one upstream activating region of the 35S CaMV promoter.
REFERENCES:
patent: 5097025 (1992-03-01), Benfey et al.
patent: 5106739 (1992-04-01), Comai et al.
patent: 5290924 (1994-03-01), Last et al.
patent: 5322938 (1994-06-01), McPherson et al.
patent: 5510474 (1996-04-01), Quail et al.
patent: 5573932 (1996-11-01), Ellis et al.
Odell et al., Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promotor, Nature, vol. 313:810-812 (1985) XP002915192.
International Search Report mailed Aug. 8, 1999.
Cornejo et al., "Activity of a Maize Ubiquitin Promoter in Transgenic Rice," Plant Molecular Biology, vol. 23, (1993), pp. 567-581.
Bowen Benjamin A.
Bruce Wesley B.
Lu Guihua
Sims Lynne E.
Tagliani Laura A.
Haas Thomas
Pioneer Hi-Bred International , Inc.
Smith Lynette R. F.
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