Plasmid capable of expressing human growth hormone

Details Plasmid capable of expressing human growth hormone Plasmid capable of expressing human growth hormone

1981-08-26

1987-01-06

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Miscellaneous

4351723, 935 9, 935 29, 935 13, C12N 100, C12N 1500

Patent

active

046346775

ABSTRACT:
Described are methods and means for the construction and microbial expression of quasi-synthetic genes arising from the combination of organic synthesis and enzymatic reverse transcription from messenger RNA sequences incomplete from the standpoint of the desired protein product. Preferred products of expression lack bio-inactivating leader sequences common in eukaryotic expression products but problematic with regard to microbial cleavage to yield bioactive material. Illustrative is a preferred embodiment in which a gene coding for human growth hormone (useful in, e.g., treatment of hypopituitary dwarfism) is constructed and expressed.

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