Method for reutilization of electrophoresis gel

Details Method for reutilization of electrophoresis gel Method for reutilization of electrophoresis gel

1988-06-24

1989-11-07

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Processes of treating materials by wave energy

2041801, 2041828, 204299R, G01N 2726

Patent

active

048790125

ABSTRACT:
An electrophoresis gel is regenerated by light-irradiating an electrophoresis gel used for separating a fluorescence-labeled sample by electrophoresis and optically detecting the sample, thereby photo-dissociating a fluorescence-labeling substance remaining in the electrophoresis gel and reutilizing the resulting gel repeatedly. A troublesome operation of preparing an electrophoresis gel at every occasion of separation and detection can be saved thereby.

REFERENCES:
patent: 4675300 (1987-06-01), Zare et al.
patent: 4729947 (1988-03-01), Middendorf et al.
Smith, Lloyd M. et al, "Fluorescence Detection in Automated DNA Sequence Analysis", Nature, vol. 321 (Jun. 12, 1986), pp. 674-679.
Peck, K. and Morris M. D., "Sensitive Photothermal Densitometer for Quantitation of Coomassie Brilliant Blue Stained Proteins in Polyacrylamide Gels", Analytical Chemistry, 1986, 58, pp. 506-507.

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