Method, device, and composition for the assay of ions

Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids

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436169, 436 79, 436171, 436172, 436805, 436810, 436530, 436531, 422 56, 422 57, 422 8205, 427 2, 427 8, G01N 2178, G01N 3352

Patent

active

053004396

ABSTRACT:
A test device and method of determining the presence or concentration of a predetermined ion, such as a cation of medical interest, like sodium, potassium, calcium, magnesium or lithium, in an aqueous test sample are disclosed. The test device includes a test pad comprising a hydrophilic indicator reagent composition capable of interacting with a predetermined ion, or electrolyte, to produce a detectable or measurable response. The hydrophilic indicator reagent composition consists essentially of a hydrophobic ion specific ionophore, a hydrophobic reporter substance, and a stabilizer polymer such as gelatin. The hydrophilic indicator reagent composition, either as a film or layer, or after incorporation into a suitable carrier matrix, provides a test pad of sufficient sensitivity to the predetermined ion to achieve an accurate and trustworthy electrolyte assay of an aqueous test sample, such as plasma or serum, by a dry phase test strip assay.

REFERENCES:
patent: 4645744 (1987-02-01), Charlton et al.
patent: 4780411 (1988-10-01), Piejko et al.

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