Counter-pressure filtration of proteins

Details Counter-pressure filtration of proteins Counter-pressure filtration of proteins

2011-06-14

2011-06-14

Fortuna, Ana M (Department: 1777)

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

C210S650000, C210S645000, C435S002000

Reexamination Certificate

active

07959809

ABSTRACT:
A method is disclosed for filtering a protein in a liquid mixture in a manner that does not substantially damage or otherwise limit the recovery of the protein in the filtration filtrate. The method generally includes passing a liquid mixture containing a protein (e.g., an aqueous vWF mixture) through a filter while applying a counter pressure to the liquid mixture filtrate to accurately reduce and control the pressure differential across the filter. The disclosed method has the advantage that relatively high filtration flow rates can be achieved at relatively low pressure differentials, in contrast to high pressure differentials, which actually reduce the filtration flow rate of protein liquid mixtures. Further, the method can recover substantially all of the protein that is initially present in the liquid mixture.

REFERENCES:
patent: 4774323 (1988-09-01), Newman et al.
patent: 5470954 (1995-11-01), Neslund et al.
patent: 5888401 (1999-03-01), Nguyen
patent: 6193891 (2001-02-01), Kent et al.
patent: 6228613 (2001-05-01), Fischer et al.
patent: 6579723 (2003-06-01), Mitterer et al.
patent: 6808638 (2004-10-01), Purdum
patent: 7439013 (2008-10-01), Kuno et al.
Truskey et al., “The Effect of Membrane Filtration Upon Protein Conformation,”J. Parenteral Science&Technology,41(6):180-93 (1987).
Letter to the Editor,J. Parenteral Science&Tecnology,vol. unknown, pp. 144-146 (ca. 1987) (discussing Truskey et al., “The Effect of Membrane Filtration Upon Protein Conformation,”J. Parenteral Science&Technology,41(6): 180-93 (1987)).
Bowen et al., “Properties of Microfiltration Membranes: The Effects of Adsorption and Shear on the Recovery of an Enzyme,”Biotechnology and Bioengineering,40:491-97 (1992).
“Filtration,”Kirk-Othmer Encyclopedia of Chem. Tech.,4thed. 10:788-853 (1993).
Siediecki et al., “Shear-Dependent Changes in the Three-Dimensional Structure of Human von Willebrand Factor,”Blood,88(8):2939-50 (1996).
Singh et al., “Effects of Fluid Shear on the Conformation of Human von Willebrand Factor Studied by Neutron and Light Scattering,” AICHE Annual Meeting paper dated Nov. 1996.
Pareti et al., “Proteolysis of von Willebrand Factor and Shear Stress-Induced Platelet Aggregation in Patients with Aortic Valve Stenosis,” Circulation, 102:1290-95 (2000).
Technical Brief, “Protein Concentration and Diafiltration by Tangential Flow Filtration,” Millipore Corporation, 25 pages (2003).
Mazurier et al., “In Vitro Study of a Triple-Secured von Willebrand Factor Concentrate,”Vox Sanguinis,86:100-04 (2004).
Schneider et al., “Shear-Induced Unfolding Triggers Adhesion of von Willebrand Factor Fibers,”PNAS,104(19):7899-903 (2007), available at www.pnas.org/cgi/doi/10.1073/pnas.0608422104.
Singh et al., “Fluid Shear Induces Conformation Change in Human Blood Protein von Willebrand Factor Solution Structure,” AICHE Annual Meeting paper dated Nov. 2007.
International Search Report and Written Opinion for Application No. PCT/US2008/088005, dated Mar. 7, 2009.
Gésen et al., “Fouling during constant flux crossflow microfiltration of pretreated whey. Influence of transmembrane pressure gradient,”Journal of Membrane Science,80:131-145 (1993).
Mietton-Peuchot et al., “Use of gas-liquid porometry measurements for selection of microfiltration membranes,”Journal of Membrane Science,133:73-81 (1997).

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