Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Reexamination Certificate
2005-06-14
2005-06-14
Spector, Lorraine (Department: 1646)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
C530S350000, C530S387900, C435S183000
Reexamination Certificate
active
06906179
ABSTRACT:
The invention provides novel compositions comprising a Smad protein and an isolated protein component of the proteasome-mediated degradation pathway. The invention also provides novel compositions comprising a Smad1 protein and a substrate for proteasome-mediated degradation. The invention also provides methods of screening for compounds that modulate the interaction between the proteins comprising these compositions. The invention also provides methods of screening for compounds that modulate the activity of the proteins comprising these compositions. The invention also provides methods of detecting proteasome-mediated degradation of novel Smad interacting proteins. A further aspect of the invention is a kit for detecting proteasome-mediated degradation of novel Smad interacting proteins. The invention also provides methods of treating diseases which are associated with aberrant levels of activity of a TGF-β superfamily member.
REFERENCES:
patent: 6063906 (2000-05-01), Brenner et al.
patent: 6139837 (2000-10-01), Bandman et al.
patent: WO98/53066 (1998-11-01), None
patent: WO 99/57132 (1999-11-01), None
patent: WO 200047102 (2000-08-01), None
Liang et al., Antibody binding to a peptide but not the whole protein by recognition of the C-terminal carboxy group, Arch. Biochem. Biophys., 329(2): 208-214 (May 15, 1996).
Lin et al., A novel link beween the proteasome pathway and the signal transduction pathway of the Bone Morphogenetic Proteins (BMPs), BMC Cell Biology 3:15 (Jun. 21, 2002).
Kawakami, T. et al.,; “NEDO human cDNA Sequencing Project”;Database Genbank; (2000) Accession: AK000340.
Nagase, et al.; “Prediction of the Coding Sequences of Unidentified Human Genes. XIII. The Complete Sequences of 100 New cDNA Clones from Brain Which Code for Large Proteins in vitro”; (1999);DNA Research; 6: 63-70.
Nagase, et al.; “Prediction of the Coding Sequences of Unidentified Human Genes. XVIII. The Complete Sequences of 100 New cDNA Clones from Brain Which Code for Large Proteins in vitro”; (2000);DNA Research; 7: 273-281.
Osman, et al.; “Identification and Characterization of a Smad2 Homologue fromSchistosoma mansomi, a Transforming Growth Factor-β Signal Transducer”; (2001);The Journal of Biological Chemistry; vol. 276, 13: 10072-10082.
Gruendler, et al.; “Proteasomal Degradation of Smad1 Induced by Bone Morphogenetic Proteins”; (2001);The Journal of Biological Chemistry; vol. 276, 49: 46533-46543.
Liu, et al.; “A novel ability of Smad3 to regulate proteasomal degradation of a Cas family member HEFI”; (2000),The EMBO Journal; vol. 19, 24: 6759-6769.
Hillier, et al.; “Generation and Analysis of 280,000 Human Expressed Sequence Tags”; (1996);Genome Research; 6: 807-828.
PCT International Search Report for International Application No.: PCT/US00/03561 dated Jan. 28, 2002.
Abdollah, et al., “TBRI Phosphorylation of Smad2 on Ser465and Ser467Is Required for Smad2-Smad4 Complex Formation and Signaling”,Journal of Biological Chemistry, 272, (1997): 27678-27685.
Baker, J. & Harland, R.M., “A Novel Mesoderm Inducer, Madr2, Functions in the Activin Signal Transduction Pathway”,Genes and Development, 10, (1996): 1880-1889.
Chen, Y., et al., “Regulation of Transforming Growth Factor Beta- and Activin-Induced Transcription by Mammalian Mad Proteins”,PNAS—Processings of the National Academy of Sciences, 93, (1996): 12992-12997.
Chen, X., et al., “Smad4 and FAST-1 in the Assembly of Activin-Responsive Factor”,Nature, 389, (1997): 85-89.
de Caestecker, M.P., et al., “Characterization of Functional Domains within Smad4 / DPC4”,Journal of Biological Chemistry, 272, (1997), 13690-13696.
Dennler, S., et al., “Direct Binding of Smad3 and Smad4 to Critical TGF-Inducible Elements in the Promoter of Human Plasminogen Activator Inhibitor-Type 1 Gene”,Embo Journal, 17, (1998): 3091-3100.
Hata, A., et al., “Mutations Increasing Autoinhibition Inactivate Tumour Supressors Smad2 and Smad4”,Nature, 388, (1997): 82-87.
Kim, J., et al., “Drosophila Mad Binds to DNA and Directly Mediates Activation of Vestigial by Decapentaplegic”,Nature, 388, (1997): 304-308.
Kretzschmar, M., et al., “The TGF-Beta Family Mediator Smad1 is Phosphorylated Directly and Activated Functionally by the BMP Receptor Kinase”,Genes and Development, 11, (1997): 984-995.
Lagna, G., et al., “Partnership Between DPC4 and SMAD Proteins in TGF-Beta Signalling Pathways”,Nature, 383, (1996): 832-836.
Liu, F., et al., “A Human Mad Protein Acting as a BMP-regulated Transcriptional Activator”,Nature, 381, (1996) 620-623.
Macias-Silva, M., et al., “MADR2 is a Substrate of the TGFbeta Receptor and Its Phosphorylation is Required for Nuclear Accumulation and Signaling”,Cell, 87, (1996): 1215-1224.
Schutte, M., et al., “DPC4 Gene in Various Tumor Types”,Cancer Research, 56, (1996): 2527-2530.
Souchelnytskyi, S., et al., “Phosphorylation of Ser465and Ser467in the C Terminus of Smad2 Mediates Interaction with Smad4 and is Required for Transforming Growth Factor-Beta Signaling”,Journal of Biological Chemistry, 272, (1997): 28107-28115.
Yingling, J. M., et al., “Tumor Suppressor Smad4 is a Transforming Growth Factor Beta-Inducible DNA Binding Protein”,Molecular and Cellular Biology, 17, (1997): 7019-7028.
Wu, R. Y., et al., “Heteromeric and Homomeric Interactions Correlate with Signaling Activity and Functional Cooperativity of Smad3 and Smad4/DPC4”,Molecular and Cellular Biology, 17, (1997): 2521-2528.
Zawel, L., et al., “Human Smad3 and Smad4 are Sequence-Specific Transcription Activators”,Molecules and Cells, 1, (1998): 611-617.
Zwickl, P., et al., “Critical Elements in Proteasome Assembly”,Nature Structural Biology, 1, (1994): 765-770.
Kaufman Claire M.
Palmer & Dodge LLP
Spector Lorraine
The General Hospital Corporation
Williams Kathleen M.
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