Δ-5 desaturases and their use in making...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

Reexamination Certificate

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C435S254110, C435S069100

Reexamination Certificate

active

07943365

ABSTRACT:
The present invention relates to Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) to arachidonic acid (ARA; 20:4 ω-6) and/or eicosatetraenoic acid (ETA; 20:4 ω-3) to eicosapentaenoic acid (EPA; 20:5 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ5 desaturases in oleaginous yeast are disclosed.

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The following applications are commonly owned by DuPont and are reported herein: U.S. Appl. No. 12/111,228, filed Apr. 29, 2008; U.S. Appl. Nos. 60/977,174, filed Oct. 3, 2007, 60/977,177, filed Oct. 3, 2007.
International Search Report and Writtten Opinion for Corresponding PCT/US2008/062196 Mailed Aug. 6, 2008.

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