Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S023100, C435S069100, C435S320100, C435S325000, C435S455000, C435S252300, C530S350000

Reexamination Certificate



The present invention is directed to novel polypeptides having homology to certain human uncoupling proteins (“UCPs”) and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention, and methods for producing the polypeptides of the present invention.

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patent: 5407810 (1995-04-01), Builder et al.
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patent: 5702902 (1997-12-01), Tartaglia
patent: 6602694 (2003-08-01), Albrandt et al.
patent: 0 307 247 (1989-03-01), None
patent: WO 98/45438 (1998-10-01), None
patent: WO 98/52958 (1998-11-01), None
Sequence alignment result 11, SEQ ID No: 2, 1-987.
Sequence alignment result 11, SEQ ID No: 2, 10-987.
Sequence alignment result 2, SEA ID No; 1 against nucleic acid database.
Sequence alignment result 6, SEQ ID No: 1 against nucleic acid database.
Sanchis et al. The J. of Biol. Chem., vol. 273. No; 51, pp 34611-34615, Dec. 18, 1998.
Albrandt et al., “Human Uncoupling Protein 4,” Database: A_Geneseq_36, Accession No. Y78511, 2000.
Altschul et al., “Local Alignment Statistics,” Methods in Enzymology, 1996, 266: 460-480.
Bolivar et al., “Construction and Characterization of New Cloning Vehicles. II. A Multipurpose Cloning System,” Gene, 1977, 2: 95-113.
Boss et al., “The Uncoupling Proteins, a Review,” European J of Endocrinology, 1998, 139(1): 1-9.
Boss et al., “Uncoupling Protein-3: a New Member of the Mitochondrial Carrier Family With Tissue-Specific Expression,” FEBS Lett, 1997, 408(1): 39-42.
Bouilland et al., “Molecular Approach to Thermogenesis in Brown Adipose Tissue: cDNA Cloning of the Mitochondrial Uncoupling Protein,” PNAS USA, 1985, 82(2): 445-448.
Cassard et al., “Human Uncoupling Protein Gene: Structure, Comparison with Rat Gene, and Assignment to the Long Arm of Chromosome 4,” J of Cellular Biochem., 1990, 43(3): 255-264.
EMBL Database: EMHUM1:AF078544 (Accession No. AF078544) 1998.
EMBL Database: EMROD:AB011068 (Accession No. AB011068) 1998.
Fleury et al., “Uncoupling Protein-2: a Novel Gene Linked to Obesity and Hyperinsulinemia,” Nature Genetics, 1997, 15(3): 269-272.
Gibson et al., “A Novel Method for Real Time Quantitative RT-PCR,” Genome Research, 1996, 6(10): 995-1001.
Gimeno et al., Cloning and Characterization of an Uncoupling Protein Homolog: A Potential Molecular Mediator of Human Thermogenesis, Diabetes, 1997, 46(5): 900-906.
Gong et al., “Uncoupling Protein-3 is a Mediator of Thermogenesis Regulated by Thyroid Hormone, β3-Adrenergic Agonists, and Leptin,” J of Bio. Chem., 1997, 272(39): 24129-24133.
Gura, “Uncoupling Proteins Provide New Clue to Obesity's Causes,” Science, 1998, 280(5368): 1369-1370.
Heid et al., “Real Time Quantitative PCR.” Genome Research, 1996, 6(10): 986-994.
Holmes et al., “Structure and Functional Expression of a Human Interleukin-8 Receptor,” Science, 1991, 253(5025): 1278-1280.
Jacobsson et al., “Mitochondrial Uncoupling Protein from Mouse Brown Fat. Molecular Cloning, Genetic Mapping, and mRNA Expression,” J of Bio. Chem., 1985, 260(30): 16250-16354.
King et al., “Phenylephrine, Endothelin, Prostaglandin F2αand Leukemia Inhibitory Factor Induce Different Cardiac Hypertrophy Phenotypes In Vitro,” Endocrine, 1998, 9(1): 45-55.
Klaus et al., “Physiology of Transgenic Mice with Brown Fat Ablation: Obesity is Due to Lowered Body Temperature,” Am. J of Physiology, 1998, 274(2 pt. 2): R287-R293.
Komarsoulis et al., “Human Secreted Protein Sequence Encoded by Gene 29, SEQ ID No. 152,” Database: A_Geneseq_36, Accession No: Y91479.
Mao et al., “UCP4, a Novel Brain-Specific Mitochondrial Protein That Reduces Membrane Potential in Mammalian Cells,” FEBS Lett., 1999, 443(3): 326-330.
NCBI/GenBank EST; Locus AA015735 (computer printout).
NCBI/GenBank EST; Locus AA015832 (computer printout).
NCBI/GenBank EST; Locus AA021118 (computer printout).
NCBI/GenBank EST; Locus AA021119 (computer printout).
NCBI/GenBank EST; Locus AA054608 (computer printout).
NCBI/GenBank EST; Locus AA056945 (computer printout).
NCBI/GenBank EST; Locus AA057005 (computer printout).
NCBI/GenBank EST; Locus AA142931 (computer printout).
NCBI/GenBank EST; Locus AA401224 (computer printout).
NCBI/GenBank EST; Locus AA404241 (computer printout).
NCBI/GenBank EST; Locus AA910774 (computer printout).
NCBI/GenBank EST; Locus AI032869 (computer printout).
NCBI/GenBank EST; Locus AI039086 (computer printout).
NCBI/GenBank EST; Locus AI128486 (computer printout).
NCBI/GenBank EST; Locus AI131262 (computer printout).
NCBI/GenBank EST; Locus AI241428 (computer printout).
NCBI/GenBank EST; Locus N48177 (computer printout).
NCBI/GenBank EST; Locus N53324 (computer printout).
NCBI/GenBank EST; Locus R19440 (computer printout).
NCBI/GenBank EST; Locus R44688 (computer printout).
Nicholls et al., “Thermogenic Mechanisms in Brown Fat,” Physiological Rev., 1984, 64(1): 1-64.
O'Reilley et al., Baculovirus Expression Vectors: A Laboratory Manual, 1994, Oxford University Press.
Ott, An introduction to Statistical Methods and Data Analysis, 1988, Boston: PWS-Kent Publishing Co.
Palou et al., “The Uncoupling Protein, Thermogenin,” Int'l. J of Biochem. & Cell Bio., 1998, 30(1): 7-11.
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Sanchis et al., “BMCP1, a Novel Mitochondrial Carrier with High Expression in the Central Nervous System of Humans and Rodents, and Respiration Uncoupling Activity in Recombinant Yeast,” J. of Bio. Chem., 1998, 273(51): 34611-34615.
Sanchis et al., “Brain Mitochondrial Carrier Protein-1 (BMCP1),” Swiss Prot_39, Accession No. O95258, 2000.
Sanchis et al., “Homo Sapiens Brain Mitochondrial Carrier Protein-1 (BMCP1),” Database, GenEmbl, Accession No. AF078544, 1999.
Solanes et al., “The Human Uncoupling Protein-3 Gene. Genomic Structure. Chromosomal, Localization, and Genetic Basis for Short and Long Form Transcripts,” J of Bio. Chem., 1997, 272(41):25433-25436.
Sompayrac et al., “Efficient Infection of Monkey Cells with DNA of Simian Virus 40,” PNAS USA, 1981, 78(12): 7575-7578.
Surwit et al., “Differential Effects of Fat and Sucrose on the Development of Obesity and Diabetes in C57BL/61 and A/1 Mice,” Metabolism, 1995, 44(5): 645-651.
Thimmappaya et al., “Adenovirus VAI RNA is Required for Efficient Translation of Viral mRNAs at Late Times After Infection,” Cell, 1982, 31(3 pt. 2): 543-551.
Vidal-Puig et al., “UCP3: An Uncoupling Protein Homologue Expressed Preferentially and Abundantly in Skeletal Muscle and Brown Adipose Tissue,” Biochem. & Biophys. Research Communications, 1997, 235(1): 79-82.
Wolf, “A New Uncoupling Protein: A Potential Component of the Human Body Weight Regulation System,” Nutrition Reviews, 1997, 55(5): 178-179.
Yu et al., “Uncoupling Protein (UCP) Homolog mRNA Abundance in Brain & Other Tissues is Modified by Nutrition & Ambient Temperature,” Experimental Biology Meeting, 1999.


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