Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Liposomes
435174, 435177, A61K 927, C12N 1100, C12N 1102
For increasing gene entrapping efficiency, it is preferable that liposome membrane to be formed is positively charged. For this purpose, a lipid with quaternary amine has been employed as one of constitutional lipids, but the such lipid may show toxicity to cells to be transformed with the gene for expression. The invention selects, as constitutional lipids for liposomes, N-(.alpha.-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), dilauroylphosphatidylcholine (DLPC), and dioleoylphosphatidylethanolamine (DOPE) with molar ratio of 1:2:2, and vortex treatment for preparing the liposomes with multi-layers to entrap the gene therein.
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Kabushiki Kaisha Vitamin Kenkyusya
Rollins John W.
Ware Deborah K.
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