Isolated DNA encoding the NotI restriction endonuclease and rela

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4353201, 43525233, 435193, 536 232, C12N 922, C12N 1555, C12N 1570

Patent

active

053710061

ABSTRACT:
The present invention is directed to a method for cloning and producing the NotI restriction endonuclease by 1) introducing the restriction endonuclease gene from Nocardia otitidis-caviarum into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the NotI restriction endonuclease activity, and 3) purifying the NotI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the NotI restriction endonuclease activity.

REFERENCES:
patent: 5202248 (1993-04-01), VanCott
Suggs, S. V., et. al. (1981) Proc. Natl. Acad. Sci., USA 78 (11) 6613-6617.
Saiki, R. K., et al. (1988) Science 239, 487-491.
Kosykh et al., Molec. Gen. Genet., 178:717-718 (1980).
Mann et al., Gene, 3:97-112 (1978).
Walder et al., Proc. Natl. Acad. Sci. USA, 78:1503-1507 (1981).
Bougueleret et al. Nucleic Acids Research, 12;3659-3676 (1984).
Gingeras & Brooks, Proc. Natl. Acad. Sci. USA, 80:402-406 (1983).
Theriault and Roy, Gene, 19:355-359 (1982).
Blumenthal et al., J. Bacteriol., 164:501-509 (1985).
Szomolanyi et al., Gene, 10:219-225 (1980).
Janulaitis et al., Gene, 20:197-204 (1982).
Kiss and Bauldauf, Gene, 21;111-119 (1983).
Walder et al., J. Biol. Chem., 258:1235-1241 (1983).
Raleigh and Wilson, Proc. Natl. Acad. Sci. USA, 83:9070-74 (1986).
Kiss et al., Nucleic Acid Research, 13:6403-6421 (1985).
Qiang and Schildkraut, Methods in Enzymology, 155:15-21 (1987).
Roberts, Nucleic Acid Research, Supp. 13:T165-r200.
Looney et al., Gene, 80:193-208 (1989).
Wilson, Methods in Enzymology 216: Recombinant DNA Part G ed. Wu, R., Academic Press, pp. 259-279 (1992).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Isolated DNA encoding the NotI restriction endonuclease and rela does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Isolated DNA encoding the NotI restriction endonuclease and rela, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolated DNA encoding the NotI restriction endonuclease and rela will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-213765

All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.