Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
424 934, 4352536, 435910, A01N 6300, C12N 112, C12N 120
The present invention relates to a new strain of an ice nucleus-forming bacterium, a process for the cultivation of the new strain of the ice nucleus-forming bacterium, an ice nucleus-forming substance containing the strain, and the uses of the ice nucleus-forming substance.
As bacteria for promoting the formation of ice, Pseudomonas syringae and Erwinia ananas have hitherto been well known. Also, among Xanthomonas campestris bacteria, those known are the following three strains: the strain obtained from the leaves of Florida citrus [Goto Masao et al., Nisshokubyo-Hou, 54, 196 (1988); and Minsavage G. V. & Stall R. E., (1986), Proc. 6th Int. Conf. Plant Path. Bact., 994-1004], X. campestris pv. translucens [Kim H. K., Orser C., Lindow S. E. and Sands D. C., Plant Disease, 71, 994-997 (1987)], and the strain obtained from tea buds (INAX) [Goto Masao et al., Nisshokubyo-Hou, 54, 189-197 (1988)].
However, among these ice nucleus-forming bacteria, Pseudomonas syringae which is utilized for practical use is known to have phytopathogenicity, so that it may adversely affect the environment when it is used for spraying to form artificial snow. On the other hand, bacteria having no such phytopathogenicity generally grow slowly and thus may not be suitable for production on an industrial scale. Additionally, bacteria which are negative with respect to tobacco hypersensitivity reaction [Phytopathology, 77, 611-615 (1987)] are generally free of phytopathogenicity.
It would therefore be extremely advantageous to the industry to discover a strain which is negative with respect to tobacco hypersensitivity reaction and yet can grow at a high rate.
An object of the present invention is to provide a new ice nucleus-forming bacterium which has the above advantages. Another object of the present invention is to provide a process for the cultivation of the new ice nucleus-forming bacterium. Still another object of the present invention is to provide an ice nucleus-forming substance containing the new ice nucleus-forming bacterium and further to develop uses thereof in various fields.
DISCLOSURE OF THE INVENTION
The present inventors have conducted extensive researches for the purpose of attaining the above stated objects. As a result, they have found that a bacterium strain obtained from tea leaves is a new strain which can fulfil the intended purpose, and have thus developed the present invention.
The present invention provides a new Xanthomonas campestris strain, INXC-1 (FERM BP-4191).
The present invention also provides a process for the cultivation of Xanthomonas campestris INXC-1.
The present invention further provides an ice nucleus-forming substance containing Xanthomonas campestris INXC-1 and presents uses thereof in various fields.
BEST MODES FOR CARRYING OUT THE INVENTION
The bacterium strain of the present invention (referred to hereinafter as "the present strain") has bacteriological properties as shown below. In this context, strains have been classified with the use of the following culture media 1-19 (numericals are based on % by weight). 7.2) 7.2) amount 0.2; bactoagar 1.5 (pH 7.2) MgSO.sub.4. 7H.sub.2 O 0.02; citric acid 0.25 (pH 6.8) indicated amount 0.05; HCl 0.02; nitrogen sources 0.1 (pH 7.2)
(Nitrogen sources are sodium nitrate and ammonium sulfate.) indicated amount indicated amount Seiyaku) MgSO.sub.4.7H.sub.2 O 0.02; bactoagar 2.0; BCP (0.2% solution) 0.4
I. Bacteriological properties
Bacillus having a bacterial cell size of 0.6-0.7 82 m.times.2-3 .mu.m, one flagellum and mobility. Negative with respect to Gram's stain. No acidophilicity. circular with smooth surface and periphery, and they are yellow, translucent and lustrous. filamentous, lustrous, yellow and translucent. layer with gelatin liquefaction. peptonized. Negative. respect to nitrates; positive with respect to ammonium salts. optimally from 23.degree. to 37.degree. C.
______________________________________ Production of acid Production of gas ______________________________________ 1. L-Ar
patent: 3413125 (1968-11-01), Schuppner et al.
patent: 4418145 (1983-11-01), Weisrock et al.
patent: 4766077 (1988-08-01), Orser et al.
Inserm Publication Honma et al., "High Pressure Sterilization of Xanthomonas campestris INXC-1 'As the Ice Nucl . . . ", 1992, Source/abstr.
Naff David M.
Q. P. Corporation
Ware Deborah K.
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