High sensitivity detection of peroxidase activity

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical


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435792, 435794, 435 28, 435805, 435970, C12Q 100, C12Q 128, G01N 3353




A new class of peroxidase substrates consists of o-diaminobenzenes having a substituent in the 4 position. The new substrates may be used in immunoassays in which a peroxidase is the label, or in assays for the peroxidase itself. Immunoassays using other enzyme labels which cause formation of hydrogen peroxide may be followed using the substrate of the invention by adding the substrate and a peroxidase to the assay fluid and detecting color formed as a result of oxidation of the substrate by the formed peroxide.

patent: 4016043 (1977-04-01), Schuurs et al.
patent: 4416982 (1983-11-01), Tsuda et al.
Tarcha, Peter J. et al., 2,3-Diaminophenazine is the Product from the Horseradish Peroxidase-Catalyzed Oxidation of o-Phenylenediamine, Analytical Biochemistry, 165, 230-233 (1987).


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