Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.
530412, A61K 3736, C07K 1300
High molecular weight forms of therapeutic proteins are disclosed which are single-polypeptide-chain proteins that contain the same or similar therapeutic activity as the therapeutic protein. In particular, high molecular weight forms of the human bFGF angiogenic factor are disclosed which are single-polypeptide-chain proteins having at least one active site possessing an activity selected from the group consisting of mitogenic activity, chemotactic activity, angiogenic activity, neurotrophic activity, the ability to stimulate protease synthesis and combinations thereof. The high molecular weight angiogenic factors exhibit substantial homology to and are immunologically equivalent to the native high molecular weight forms isolatable from human hepatoma cells. The high molecular weight angiogenic factors are produced by DNA translation initiating at non-ATG codons and incorporate additional polypeptide sequences N-terminal to the human bFGF factor. The amino acid sequences of the high molecular weight angiogenic factors and the oligonucleotide sequence that translate to form the high molecular weight bFGF proteins are also disclosed. Methods for isolating the high molecular weight angiogenic factors from human hepatoma cell line SK-HEP-1 and producing the proteins by recombinant DNA techniques are also described.
Klagsburn et al. Proc. Natl Acad Sci USA 84:1839-1843 (1987).
Abraham et al. The Embo J. 5(10):2523-28 (1986).
Presta et al. Molecular & Cellular Biol. 6(11):4060-66 (1986).
Hann et al. Cell 52:185-195 (Jan. 29, 1988).
Sommer et al. Biochem & Biophys Res Comm 144(2):543-50 (1987).
Moscatelli et al. Proc Natl Acad Sci USA 83:2091-95 (1986).
Klagsburn et al. Proc Natl Acad Sci USA 83:2448-52 (1986).
Moscatelli et al Proc Natl Acad Sci USA 84:5778-82 (1987).
Moscatelli et al Biochimie 70:83-87 (Jan., 1988).
Ueno et al. Biochem Biophys Res Comm 138(2):580-88 (1986).
Klagsburn et al. in Angiogenesis Mechanisms & Pathobiology ed. Rifkin, D. B., Cold Spring Harbor:1987.
Kurokawa et al. FEBS 213(1):189-194 (1987).
Bowie et al. Science 247:1306-1310 (1990).
Florkiewicz Robert Z.
Cermak Shelly Guest
Hill Jr. Robert J.
High molecular weight human angiogenic basic fibroblast growth f does not yet have a rating. At this time, there are no reviews or comments for this patent.If you have personal experience with High molecular weight human angiogenic basic fibroblast growth f, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High molecular weight human angiogenic basic fibroblast growth f will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1053835