Directional cloning

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 91, 4353201, 935 18, 935 26, 935 27, 935 29, 935 31, 935 56, 935 58, C12N 1500, C12N 1510, C12N 1570, C12P 1934

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050752278

ABSTRACT:
Methods for cloning cDNA and producing mRNA from the cloned cDNA are disclosed. The cDNA is cloned in one orientation through the use of a vector containing a directional cloning site and through the use of primer-adapter sequences complementary to portions of the directional cloning site. The vector may also contain a promoter, one or more terminators and a polyadenylation signal to facilitate transcription of the cloned cDNA and translation of the mRNA.

REFERENCES:
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