Directional cloning

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses


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435 91, 4353201, 935 18, 935 26, 935 27, 935 29, 935 31, 935 56, 935 58, C12N 1500, C12N 1510, C12N 1570, C12P 1934




Methods for cloning cDNA and producing mRNA from the cloned cDNA are disclosed. The cDNA is cloned in one orientation through the use of a vector containing a directional cloning site and through the use of primer-adapter sequences complementary to portions of the directional cloning site. The vector may also contain a promoter, one or more terminators and a polyadenylation signal to facilitate transcription of the cloned cDNA and translation of the mRNA.

patent: 4342832 (1982-08-01), Goeddel et al.
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