Cloning restriction and modification genes

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435193, 435199, C12N 1555

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active

052003337

ABSTRACT:
Methods for cloning restriction enzymes and their corresponding modification enzymes by selecting clones resistant to digestion by the restriction enzyme and subsequent selection of clones containing the restriction gene.

REFERENCES:
patent: 4530904 (1985-07-01), Hershberger et al.
Mann, et al. Cloning of Restriction and Modification Genes, vol. 3, No. 2 pp. 97-112 (1978).
Szomolanyi, et al. Gene, "Cloning the Modification Methylase Gene of Bacillus sphaericus R " in E. coli, vol. 10, pp. 219-225 (1980).
Walder, et al. Proc. Natl. Acad. Sci. USA "Cloning and Expression of the Pst I restriction-modification system in E. coli", vol. 78, No. 3, pp. 1503-1507 (1981).
Janulaitis, et al. Gene, "Cloning of the Modification of Methylase Gene of Bacillus centrosporus in E. coli", vol. 20, pp. 197-204 (1982).
Walder, et al. Journal of Biochemistry, "Cloning of the MspI Modification Enzyme", vol. 258, pp. 1235-1241 (1983).
Nucleic Acids, Res. 10, 5745-5752.
Nucleic Acids Res. 7 1513 (1979). Sato, Showbu et al, "A Thermostable sequence-specific endonuclease from Thermus aquaticus". Proc. Natl. Acad. Sci. USA vol. 74, No. 2, pp. 542-546 (Feb. 1977).
Tu; Chen-Pei D. et al "Nucleotide Recognition Sequence at the Cleavage Site", Biochemical and Biophysical Research Communciations vol. 72 No. 7, pp. 355-362 (1976).
Sato, Showbu et al. "A DNA Methylase from Thermus thermophilus HB8",J. Biochem. vol. 88, pp. 737--747 (1980).
Proc. Nat. Acad. Sci. 78 1503-1507 (1981).

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