Cloning restriction and modification genes

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses


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435193, 435199, C12N 1555




Methods for cloning restriction enzymes and their corresponding modification enzymes by selecting clones resistant to digestion by the restriction enzyme and subsequent selection of clones containing the restriction gene.

patent: 4530904 (1985-07-01), Hershberger et al.
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Walder, et al. Proc. Natl. Acad. Sci. USA "Cloning and Expression of the Pst I restriction-modification system in E. coli", vol. 78, No. 3, pp. 1503-1507 (1981).
Janulaitis, et al. Gene, "Cloning of the Modification of Methylase Gene of Bacillus centrosporus in E. coli", vol. 20, pp. 197-204 (1982).
Walder, et al. Journal of Biochemistry, "Cloning of the MspI Modification Enzyme", vol. 258, pp. 1235-1241 (1983).
Nucleic Acids, Res. 10, 5745-5752.
Nucleic Acids Res. 7 1513 (1979). Sato, Showbu et al, "A Thermostable sequence-specific endonuclease from Thermus aquaticus". Proc. Natl. Acad. Sci. USA vol. 74, No. 2, pp. 542-546 (Feb. 1977).
Tu; Chen-Pei D. et al "Nucleotide Recognition Sequence at the Cleavage Site", Biochemical and Biophysical Research Communciations vol. 72 No. 7, pp. 355-362 (1976).
Sato, Showbu et al. "A DNA Methylase from Thermus thermophilus HB8",J. Biochem. vol. 88, pp. 737--747 (1980).
Proc. Nat. Acad. Sci. 78 1503-1507 (1981).


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