Cloning restriction and modification genes

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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4352523, 43525233, 4353201, 536 232, C12N 922, C12N 1555

Patent

active

051806738

ABSTRACT:
Methods for cloning restriction enzymes and their corresponding modification enzymes by selecting clones resistant to in vitro digestion by the restriction enzyme and subsequent screening to identify those clones containing the restriction gene.

REFERENCES:
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Mann, M. B., et al. (1978) Gene 3, 97-112.
Sato et al Proc Natl Acad Sci vol. 74 (2) pp. 542-546, Feb. 1977 "Athermpstable Seqence-Specific Endonuclease".
Sato et al J Biochem vol. 88, pp. 737-747 1980, "A DNA Methylase from Thermos thermophilus HB8".
Tu et al. Biochem Biophys Res Comm vol. 72 (7) pp. 355-362, 1976 "Nucleotide Recognitive Sequence at the Cleavage Site".
Walder et al J Biol Chem vol. 258 pp. 1235-1241 1983.
Szomolanyi et al Gene vol. 10 pp. 219-225 1980.
Janulaitis et al Gene vol. 20 pp. 197-204 1982.

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