Cell culture medium with antioxidant

Chemistry: molecular biology and microbiology – Spore forming or isolating process


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4352402, 43524025, 43524026, 4352403, 435244, C12N 500, C12N 502, C12N 138




Cells grown in chemically defined (serum free) media are protected against endogenous oxidizing agents by a nontoxic antioxidant with free thiol groups, at cell densities less than about 10.sup.5 cells/ml, especially DL-penicillamine, N-acetylcysteine, mercaptoproprionic acid, 2-mercaptoethanesulfonic acid, and thiolactate. These agensts are useful in the maintenance and growth of hybridomas in such media.

Darfler et al., J. Cell Phys., 115:31-36 (1983), "Clonal Growth of Lymphoid Cells in Serum-Free Media Requires Elimination of H.sub.2 O.sub.2 Toxicity".
Broome et al., J. Exp. Med., 138: 574-592 (1973), "Promotion of Replication in Lymphoid Cells by Specific Thiols and Disulfides in Vitro".


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