Bioadhesives for cell and tissue adhesion

Chemistry: molecular biology and microbiology – Spore forming or isolating process

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4352401, 4352402, 43524021, 43524023, 435174, 435177, 435180, C12N 500, C12N 1100

Patent

active

051089238

ABSTRACT:
A cell culturing system, methods for the preparation thereof, and methods for affixing other biologically active moieties to a substrate are provided. Said cell culturing system comprises: a substrate; a coating thereon of a sterile formulation comprising polyphenolic protein containing from about 35 to 100% by weight pure bioadhesive polyphenolic protein having the repeating decapeptide unit: ##STR1##
wherein N is a whole number ranging from about 10 to about 100, wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, and wherein each R is independently selected from the group consisting of hydrogen and methyl; viable cells affixed to said coated substrate; and a nutritive medium contacting said cells, whereby said cells perform normal metabolic cell functions.

REFERENCES:
patent: 3910819 (1975-10-01), Rembaum et al.
patent: 4119589 (1978-10-01), Horn et al.
patent: 4266032 (1981-05-01), Miller et al.
patent: 4352887 (1982-10-01), Reid et al.
patent: 4496397 (1985-01-01), Waite
patent: 4501815 (1985-02-01), Reid et al.
patent: 4553974 (1985-11-01), Dewanjee
patent: 4559304 (1985-12-01), Kasai et al.
patent: 4578079 (1986-03-01), Rouslahti et al.
patent: 4585585 (1986-04-01), Waite
Picciano et al, "Mussel Adhesive Protein: A New Cell Attachment Factor", In Vitro: Cellular and Developmental Biology, vol. 22(3), p. 24A, Ab #55, Mar. 1986.
Biopolymers, Inc., "Cell-Tak", Dec. 1986.
Biological Abstracts, vol. 77(3), #19656, Mills et al, 1983.
Chemical Abstracts, vol. 100, #56753y, Illum et al, 1984.
Gospodarowicz et al., "The Role of the Extracellular Matrix and Growth Factors in the Control of Proliferation of Anchorage-Dependent Cells", Prog. Cancer Res. Ther., vol. 23, 73-104, 1982.
Alitalo et al, "Extracellular Matrix Proteins of Human Epideraml Keratinocytes and Feeder 3T3 Cells", J. Cell. Biol., vol. 94, 497-505, 1982.
Foxall et al, "Adult Human Endothelial Cell Coverage of Small Caliber Dacron and Polytetrafluorolkylene Vascular Prosthesis In-Vitro", J. Surg. Res. vol. 41(2), 158-172, 1986.
Leivo et al, "Basal Lamina Glycoproteins Laminin and Type IV Collagen are Assembled into a Fine-Fibered Matrix in Cultures of a Teratocarcinoma-Derived Endodermal Cell Line", Exp. Cell Res., vol. 137(1), 15-23, 1982.
Hawrot et al., "Long-Term Culture of Dissociated Sympathetic Neutrons", Methods in Enzymology, vol. LVIII, pp. 574-584, 1979, Jakoby et al., ed. Academic Press.
Imagawa et al, "Isolation and Serum-Free Cultivation of Mammary Epithelial Cells within a Collagen Gel Matrix", Methods for Serum-Free Culture of Cells of the Endocrine System, Barnes et al., ed., pp. 127-441, 1984, Alan R. Liss, Inc., New York, N.Y.
Waite et al, "Polyphenolic Substance of Mytilus Edulis: Novel Adhesive Containing L-Dopa and Hydroxyproline", Science, vol. 212, pp. 1038-1040, 1981.
Collaborative Research, Inc. (Nov. 1983).
Bethesda Research Laboratories (1981/1982).
Sigma Chemical Company (Feb. 1985).
Biomatrix Product, pp. 10-13.
CalBioChem, "Extracellular Matrix Protein Research Aided by CalBioChem Antibodies".
Sigma, "Tissue Culture Media and Reagents".
Telios, "Telios Pharmaceuticals Research Products".
McKeehan and Ham, "Stimulation of Clonal Growth of Normal Fibroblasts with Substrata Coated with Basic Polymers," J. Cell Biol. 71:727-734 (1976).
Waite, "Quinone-Tanned Schleroproteins" in 1 The Mollusca, 467-504 (1981).
Science, vol. 212, 1038-1040 (May 29, 1981).
"Nature's Underwater Adhesive Specialist", Int. J. Adhesion and Adhesives, vol. 7, NO. 1, 9-14 (Jan. 1987).
"Novel Materials from Protein-Polymer Grafts", Nature, vol. 325, 328-329 (Jan. 22, 1987).
"Mussel Glue From Mytilus californianus Conrad: A Comparative Study", Journal of Comparative Physiology B, vol. 156, 491-496 (1986).
"Assay of Dihydroxyphenlalanine (dopa) in Invertebrate Structural Proteins", Methods in Enzymology, vol. 107, 397-413 (1984).
"Catechol Oxidase in the Byssus of the Common Mussel", Mytilus edulis L., J. Mar. Biol. Ass. U.K., vol. 65, 359-371 (1985).
"Evidence for a Repeating 3,4-Dihydroxyphenylalanine- and Hydroxyproline-Containing Decapeptide in the Adhesive Protein of the Mussel, Mytilus edulis L.", Journal of Biological Chemistry, vol. 258, No. 5, 2911-2915 (Mar. 10, 1983).
"Peptide Repeats in a Mussel Glue Protein: Theme and Variations", Biochemistry, vol. 24, 5010 (1985).
Ito et al, "Oxidation of Tyrosine Residues in Proteins by Tyrosinase", Biochem. J., vol. 222, 407-411 (1984).
Ruoslahti et al., "Synthetic Peptides Causing Cellular Adhesion to Surfaces," Biotechniques, 38-41 (Jan./Feb. 1984).
Cook, M., "Composition of Mussel and Barnacle Deposits at the Attachment Interface," Adhesion in Biologicals Systems, 139-150 (Academic Press) (1970).
Gross and Hoffman, "Medical and Biological Adhesives," Handbook of Adhesives, 818-835 (2d. ed., van Nostrand Reinhold Co.) (1977).
Pierschbacher & Rouslahti, "Cell-Attachment Activity of Fibronectin can be Duplicated by Small Synthetic Fragments of the Molecule", Nature, vol. 309, No. 5963, pp. 30-33, (May 1984).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Bioadhesives for cell and tissue adhesion does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Bioadhesives for cell and tissue adhesion, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bioadhesives for cell and tissue adhesion will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1248927

All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.