Assay to detect the presence of live virus in vitro

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage


Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0


435 29, 435236, 436820, 424 89, C12Q 170, C12Q 102, C12N 704, A61K 3912




An assay to detect the presence of live hepatitis viruses in vitro. Bone marrow cells of leukemic cell line cells are exposed to a body fluid or biological preparation to be tested and the cells are placed in suspension. When using bone marrow cells, growth factors to the bone marrow stem cells are added. It has been determined that presence of a live hepatitis virus suppresses the growth of colonies of the stem cells. Therefore, if the number of colonies growing in the mixture are less than that number present in a culture of cells exposed to a sample that has been determined to contain no live virus, live hepatitis virus is present in the sample tested. The assay is particularly useful to determine the presence of live hepatitis B virus in a vaccine.

patent: 4230697 (1980-10-01), Nishida et al.
patent: 4783407 (1988-11-01), Provost et al.
patent: 4952494 (1990-08-01), Zeldis et al.
Zeldis et al., J. Clin. Invest., vol. 78, 1986, pp. 411-417.
Yoffe et al., J. Infect. Dis., 153(3), 471-477 (1986).
Pontisso et al., Br. Med. J., 288: 1563-1566 (1984).
Elfassi et al., Proc. Nat'l Acad. Sci. USA, vol. 81, pp. 3526-3528 (1984).
Lie-Injo et al., DNA, 2(4), 301-307 (1983).
Laine et al., Science (USA), 229: 561-563 (1985).
Romet-Lemonne et al., Science, vol. 221, pp. 667-669, Aug. 1983.
Tabor et al., J. Med. Virol., 11(1), 1983, pp. 1-10.
Alberts et al., Molecular Biology of the Cell, 1983, Garland Publishing, Inc., pp. 921-924.
Romet-Lemonne et al., "Hepatitis B Virus Infection in Cultured Human Lymphoblastoid Cells", Science, vol. 221 (Aug. 1983).
Young et al., "Characterization of a Virus that Causes Transient Aplastic Crisis", The Journal of Clinical Investigation, Inc., vol. 73 (Jan. 1984).
J. B. Zeldis, H. Mugishima, R. P. Gale, H. Steinberg, "Hepatitis B Virus Inhibition of Bone Marrow Stem Cells", Gastroenterology, vol. 90, May 1986, p. 1783.


Say what you really think

Search for the USA inventors and patents. Rate them and share your experience with other people.


Assay to detect the presence of live virus in vitro does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Assay to detect the presence of live virus in vitro, we encourage you to share that experience with our community. Your opinion is very important and Assay to detect the presence of live virus in vitro will most certainly appreciate the feedback.

Rate now


Profile ID: LFUS-PAI-O-835165

All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.