2′ Modified oligonucleotides

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S025300, C536S026260, C536S025340, C536S025600, C536S026700

Reexamination Certificate



Oligomers which have substituents on the 2′ position are resistant to oligonucleases and furthermore can be derivatized to deliver reagents or drugs, to carry label, or to provide other properties.

patent: 4445066 (1984-04-01), Nishiyama et al.
patent: 4458066 (1984-07-01), Caruthers et al.
patent: 4476301 (1984-10-01), Imbach et al.
patent: 4711955 (1987-12-01), Ward et al.
patent: 4828979 (1989-05-01), Klevan et al.
patent: 4849513 (1989-07-01), Smith et al.
patent: 4924624 (1990-05-01), Suhadolnik et al.
patent: 4981957 (1991-01-01), Lebleu et al.
patent: 5013830 (1991-05-01), Ohtsuka et al.
patent: 5109126 (1992-04-01), Agrawal et al.
patent: 5264423 (1993-11-01), Cohen et al.
patent: 5792847 (1998-08-01), Buhr et al.
patent: 6476205 (2002-11-01), Buhr et al.
patent: 0 267 996 (1986-11-01), None
patent: 0 269 574 (1988-06-01), None
patent: 0 339 842 (1989-11-01), None
patent: 0 679 657 (1995-11-01), None
patent: 0 339 842 (1996-11-01), None
patent: WO 86/05518 (1986-09-01), None
patent: WO 88/00201 (1988-01-01), None
patent: WO 89/05853 (1989-06-01), None
Alderfer, et al., “Comparative Studies on Homopolymers of Adenylic Acid Possessing Different C-2′ Substituents of the Furanose. Poly(deoxyriboadenylic acid), Poly(riboadenylic acid), Poly(2′-0-methyladenylic acid), and Poly(2′-0-ethyladenylic acid)”,Biochem,1974, 13(8), 1615-1622.
Andrus, et al., “Novel Activating and Capping Reagents for Improved Hydrogen-Phosphonate DNA Synthesis”,Tet Lett,1988, 29(8), 861-864.
Arnott, et al., “Structures for the Polynucleotide Complexes Poly(dA). Poly(dT) and Poly(dT). Poly(dA).Poly(dT)”J. Mol. Biol.,1974, 88, 509-521.
Baum, R.M., “Mechanism of Sequence-Specific DNA Recognition Elucidated”,C&E News,1988, 20-26.
Bobst, et al., “Role of the Ribose 2′-Hydroxyl Groups for the Stabilization of the Ordered Structures of Ribonucleic Acid”,J Am Chem Soc.,1969, 91(16), 4603-4604.
Chavis, et al., “Synthesis of 2′, 3′-Differentiated Ribonucleosides via Glycosylation Reaction with 2-O-Me or 2-O-TBDMS Ribofuranose Derivatives. 1. Pyrimidine Series”,J. Org. Chem.,1982, 47, 202-206.
Chladek, et al., “Oligonucleotide Compounds. VIII. Synthesis of Adenyly-(5′->3′)-Uridine, Adenylyl-)5′->3′)-Cytidine, Guamylyl-(5′->3″)-Uridine, Guanylyl-(5′->3′-Cytidine, 0Cytidylyl-(5′->3′)-Cytidine Adenylyl-(5′->3′)-Uridylyl-(5′->3′) . . . ”,Collect Chem Commun.,1964, 29, 214-233.
Chladek, et al., “Oligonucleotide Compounds XII. Synthesis of Cytidylyl-(3′->5′)-Cytidylyl-(3′->5′)-Adenosine (CpCpA), an acceptor Sequence of Soluble Ribonucleic Acid”,Collect Czech Chem Commun,1996, 31, 1785-1802.
Chollet, et al., “DNA containing the base analogue 2-aminoadenine: preparation , use as hybridization probes and cleavage by restriction endonucleases”,Nuc Acids Res,1988, 16(1), 305-317.
Clontech,Advertisement,1988, 16(22).
Cooney, et al., “Site-Specific Oligonucleotide Binding Represses Transcription of the Human c-myc Gene In Vitro”,Science,1988, 241, 456-459.
Cummins, et al., “Characterization of fully 2′-modified oligoribonucleotide hetero- and homoduplex hybridization and nuclease sensitivity”,Nuc. Acids Res,1995, 23(11), 2019-2024.
Dervan, P.B., “Sequence-specific recognition of double helical DNA. A Synthetic Approach”,Nuc. Acids and Molecular Biology,1988, 2, 49-64.
Divaker, et al., “Reaction Between 2, 2′-Anhydro-1-beta-o-arabinofuranosyluracil and Thiolate Ions”,J Chem Soc Perkin Trans I,1982, 1625-1628.
Francois, et al., “Sequence-specific recognition of the major groove of DNA by oligodeoxynucleotides via triple helix formation. Footprinting studies”, Nuc Acids Res, 1988, 16, 11431-11440.
Froehler, et al., “Deoxynucleoside H-Phosphonate Diester Intermediates in the Synthesis of Internucleotide Phosphate Analogs”,Tet Lett,1986, 27, 5575-5578.
Froehler, et al., “The Use of Nucleoside H-Phosphonates in the Synthesis of Deoxyoligonucleotides”,Nucls&Nuclt,1987, 6, 287-291.
Fukukawa et al., “Studies on the Synthesis of Pyrimidine Deoxynucleosides. I. Synthesis of 2′,3′-Dideoxyuridine and 1-(3-Ethylthio-3-deoxy-beta-D-xylofuranosyl) uracil”,Chem Pharm Bull,1970, 18, 554-560.
Fukukawa, et al., “Synthesis of 2′(R)-Substituted Neplanocin A's (Nucleosides and Nucleotides. XXXVII)”, 1981, 29(2), 597-600.
Griffin, et al., “Recognition of Thymine-Adenine Base Pairs by Guanine in a Pyrimidine Triple Helix Motif”,Science,1989, 245, 967-971.
Griffin, et al., “Some Observations Relating to Acyl Mobility in Aminoacyl Ribonucleic Acids”,Biochem,1966, 5(11), 3638-3649.
Hobbs, et al., “Polynucleotides containing 2′-chloro-2-deoxyribose”, 1972,Biochem,11(23), 4336-4344.
Hobbs, et al., “Polynucleotide Containing 2-Amino-2′-deoxyribose and 2′-Azido-2′-deoxyribose”,Biochem,1973, 12, 5138-5145.
Hobbs, et al., “Poly 2′-Deoxy-2′-Aninouridylic Acid”,Biochem Biophys Res Comm,1972, 46(4), 1509-1515.
Inoue, et al., “Synthesis and hybridization studies on two complementary nona(2′-methyl) ribonucleotides”,Nuc Acids Res,1987, 15-6131-6148.
Inoue, et al., “Synthesis and properties of novel nucleic acid probes”,Nuc Acids Res.,1985, 16, 165-169.
Inoue, et al., “Sequence-dependent hydrolysis of RNA using modified oligonucleotide splints and Rnase H”,FEBS,1987, 215(2), 327-330.
Iribarren, et al., “2″-O-Alkyl oligoribonucleotides as antisense probes”,Proc Natl Acad Sci,1990, 87, 7747-7751.
Izant, et al., “Constitutive and Conditional Suppression of Exogenous and Endogenous Genes by Anti-Sense RNA”,Science,1985, 229, 345-352.
Janik, et al., “Synthesis and Properties of Poly 2′-Fluoro-2′-Deoxyuridylic Acid”,Biochem Biophys Res Commun ,1972, 46(3), 1153-1160.
Jencks, W.P. et al., “Preparation and Properties of Acyl Adenylates”,Methods In Enzymology,1959, 6, 762-766.
Johnston, G.A. R., “Acetylation of Nucleosides and Acetyl Migration”,Tetrahedron,1968, 24, 6987-6993.
Kaufmann, et al., “Phosphorolysis of Aminoacyl-tRNA by Polynucleotide Phosphorylase fromEscherichia coli”, Eur J. Biochem,1970, 12, 85-92.
Kaufmann, et al., “Monofunctional Substrates of Polynucleotide Phosphorylase”,Eur J. Biochem,1971, 24, 4-11.
Keller, et al., “Synthesis and hybridization properties of oligonucleotides containing 2′-O-modified ribonucleotides”,Nuc Acids Res,1993, 21(19), 4499-4505.
Khurshid, et al., “The Unique Conformational Stability of Poly 2′-0-Ethyladenylic Acid”,FEBS,1972, 28(1), 25-28.
Kielanowska, et al., “Preparation and Properties of Poly 2′-O-Ethylcytidylic Acid”,Nuc Acids Res,1976, 3(3), 817-824.
Kusmeirek, et al., “Alkylation of Cytidine-5′-Phosphate: Mechanisms of Alkylation, Influence of O' Alkylation on Susceptibility of Pyrimidine Nucleotides to Some Nucleolytic Enzymes, and Synthesis of 2′-O-Alkyl Polynucleotides”,Acta Biochimica Polonica,1973, 20(4), 365-381.
Lamond, et al., “Antisense oligonucleotides made of 2′-O-alkylRNA: their properties and applications in RNA biochemistry”,FEBS,1993, 325(1,2), 123-127.
Lapidot, et al., “Studies on Polynucleotides. XXVII. The Specific Synthesis of C3-C5,-Linked Ribooligonucleotides(4). The Stepwise Synthesis of Uridylyl-(3->5′)-uridylyl-(3″->5′)-uridine”,J Am Chem Soc.,1963, 85, 3852-3857.
Larock, R.C. “Comprehensive Organic Transformations: a guide to functional grou


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